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Metabolism Lipase Atglistatin

Atglistatin

Catalog No. T1875   CAS 1469924-27-3
Purity 99.81% Datasheet MSDS

Atglistatin is a highly effective, specific and competitive ATGL inhibitor with an IC50 of ~0.7 μM for inhibition of lipolysis in vitro, and no toxicity when the concentration up to 50 μM.

Atglistatin, CAS 1469924-27-3
Pack Size Availability Price/USD Quantity
2 mg In stock 48.00
5 mg In stock 68.00
10 mg In stock 122.00
25 mg In stock 227.00
50 mg In stock 410.00
100 mg In stock 648.00
1 mL * 10 mM (in DMSO) In stock 74.00
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Atglistatin is a highly effective, specific and competitive ATGL inhibitor with an IC50 of ~0.7 μM for inhibition of lipolysis in vitro, and no toxicity when the concentration up to 50 μM.
Targets&IC50 ATGL :ic50 0.7μM,  
Kinase Assay Determination of lipase activity: For determination of lipase activity, lysates are incubated with a substrate containing radiolabeled [9,10-3H(N)]-triolein. Subsequently, FA are extracted and quantitated by liquid scintilation counting. Data are presented as mean ?S.D. of triplicate determinations and representative for at least three independent experiments.
Cell Research
For MTT-based in vitro viability assays, cells are seeded in 96-well plates and cultured under standard conditions for 24 h. The next day, cells are pretreated with different concentrations of Atglistatin dissolved in DMSO or Cisplatin dissolved in DMF as positive control for 2 h. Medium is replaced by an identical fresh medium and incubated again for the indicated time points. Thereafter, cells are incubated for 3 h with 100 μL Thiazolyl Blue Tetrazolium Bromide (MTT). The resulting violet formazan crystals are dissolved by adding 100 μL of MTT solubilization solution (0.1% NP-40, 4 mM HCl and anhydrous isopropanol). After complete dissolution of the formazan product, absorbance is measured at 595 nm using 690 nm as reference wavelength.(Only for Reference)
Cell lines: AML-12 mouse hepatocytes
Animal Research
Animal Model: Mice (C57Bl/6J)
Appearance solid
Molecular Weight 283.37
Formula C17H21N3O
CAS No. 1469924-27-3

Storage

-20℃ 3 years powder

-80℃ 2 years in solvent

Solubility Information

DMSO: 52 mg/mL (183.5 mM)

Ethanol: 4 mg/mL (14.11 mM)

Water: <1 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Mayer N, et al. Nat Chem Biol. 2013, 9(12), 785-787. 1. Mayer N, et al. Nat Chem Biol. 2013, 9(12), 785-787.

Related Compound Libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Bioactive Lipid Compound Library Anti-Metabolism Disease Compound Library

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