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CVT-313

Catalog No. T3195   CAS 199986-75-9
Synonyms: CVT 313, Cdk2 Inhibitor III, CVT313, NG26

CVT-313 (NG-26) is a potent, selective, reversible, and ATP-competitive inhibitor.

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CVT-313 Chemical Structure
CVT-313, CAS 199986-75-9
Pack Size Availability Price/USD Quantity
1 mg In stock $ 37.00
2 mg In stock $ 50.00
5 mg In stock $ 80.00
10 mg In stock $ 121.00
25 mg In stock $ 219.00
50 mg In stock $ 400.00
100 mg In stock $ 595.00
1 mL * 10 mM (in DMSO) In stock $ 80.00
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Purity: 97.82%
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Biological Description
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Description CVT-313 (NG-26) is a potent, selective, reversible, and ATP-competitive inhibitor.
Targets&IC50 CDK2:0.5 μM
In vitro CVT-313 has been shown to inhibit other kinases, but at much higher IC50 values, i.e., CDK1 (IC50=4.2 μM), CDK4 D1 (IC50=215 μM), and MAPK/PKA/PKC (IC50>1.25 mM), compared to CDK2 (IC50=0.5 μM). CVT-313 has been shown to have profound effects on cell proliferation at concentrations of 5-20 μM[1]. CVT-313 is a potent CDK2 inhibitor, which is identified from a purine analog library with an IC50 of 0.5 μM in vitro. Inhibition is competitive with respect to ATP (Ki=95 nM), and selective CVT-313 has no effect on other, nonrelated ATP-dependent serine/threonine kinases. When added to CDK1 or CDK4, a 8.5- and 430-fold higher concentration of CVT-313 is required for half-maximal inhibition of the enzyme activity. Using normal and tumor human/murine cell lines, the effects of CVT-313 on cell proliferation is measured. The IC50 for growth inhibition ranged from 1.25 to 20 μM[2].
Kinase Assay For kinase assays, purified CDC5L(295-795)-His6 is mixed with [γ-32P]ATP, COS-7 cell extract, and incubated in 100 μL 20 mM HEPES, pH 7.5, 50 mM NaCl, 2 mM MnCl2, 10 mM MgCl2, 0.5% NP-40, 0.5 mM PMSF, 5 mM benzamidine hydrochloride, 5 mM NaF, 1 mM NaVO3 and the specific inhibitor at 30°C for 10 minutes. Cell extract as a source of kinase activity is prepared from subconfluent, serum-stimulated COS-7 cells lysed in 20 mM HEPES-NaOH, pH 7.5, 50 mM NaCl, 1% Triton X-100, 10% glycerol, protease and phosphotase inhibitors. Phosphorylated proteins are separated by electrophoresis in 15% polyacrylamide-SDS gels. Specific inhibitors included 20 μM staurosporine, 10 μM genistein, 1 μM CVT-313, 10 μM Rp-MB-cAMPS and 50 μM PD98059[1].
Cell Research CVT313 is prepared in DMSO and stored, and then diluted with appropriate medium before use[2]. MRC-5 cells are grown in Dulbecco's modified Eagle's medium containing 5% fetal calf serum. CVT313 (0, 5, 10, 15 μM) is added to exponentially growing cells in tissue culture. Cell population is measured. Proliferation assays are carried out using the nonradioactive CellTiter 96 kit after 48-h exposure. For FACS analysis of DNA content, cells are trypsinized, fixed in 70% ice-cold ethanol, and treated with 0.1 mg/mL RNase A and 40 μg/mL propidium iodide for 1 h at 37°C[2].
Synonyms CVT 313, Cdk2 Inhibitor III, CVT313, NG26
Molecular Weight 400.47
Formula C20H28N6O3
CAS No. 199986-75-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 50 mg/mL (124.85 mM)

TargetMolReferences and Literature

1. Graub R, et al. Cell cycle-dependent phosphorylation of human CDC5 regulates RNA processing. Cell Cycle. 2008 Jun 15;7(12):1795-803. 2. Brooks EE, et al. CVT-313, a specific and potent inhibitor of CDK2 that prevents neointimal proliferation. J Biol Chem. 1997 Nov 14;272(46):29207-11.

TargetMolCitations

1. Jiang L, Yu Y, Li Z, et al.BMS-265246, a Cyclin-Dependent Kinase Inhibitor, Inhibits the Infection of Herpes Simplex Virus Type 1.Viruses.2023, 15(8): 1642.

Related compound libraries

This product is contained In the following compound libraries:
NO PAINS Compound Library Anti-Pancreatic Cancer Compound Library Inhibitor Library Anti-Breast Cancer Compound Library Anti-Cancer Compound Library Bioactive Compound Library Preclinical Compound Library Anti-Prostate Cancer Compound Library Bioactive Compounds Library Max Anti-Cardiovascular Disease Compound Library

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Keywords

CVT-313 199986-75-9 Cell Cycle/Checkpoint CDK inhibit CVT 313 Cdk2 Inhibitor III CVT313 Cyclin dependent kinase NG-26 NG 26 NG26 Inhibitor inhibitor

 

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