Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Genistein (NPI 031L) is an isoflavonoid derived from soy products. It inhibits protein-tyrosine kinase and topoisomerase-II (DNA TOPOISOMERASES, TYPE II), with antineoplastic and antitumor activities.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 mg | In stock | $ 38.00 | |
100 mg | In stock | $ 54.00 | |
500 mg | In stock | $ 96.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 57.00 |
Description | Genistein (NPI 031L) is an isoflavonoid derived from soy products. It inhibits protein-tyrosine kinase and topoisomerase-II (DNA TOPOISOMERASES, TYPE II), with antineoplastic and antitumor activities. |
Targets&IC50 | EGFR:0.6 μM (IC50, Cell Assay) |
Kinase Assay | Purified FXa is obtained after activation with Russell's viper venom followed by affinity chromatography. The resulting FXa is > 95% pure as judged by sodium dodecylsulfate polyacrylamide gel electrophoresis. The substrate affinity values for FXa, expressed as the Michaelis-Menten-Henri constant (Km), for human, rabbit, rat and dog FXa are determined using the chromogenic substrate S-2765, and are 36, 60, 240 and 70 μM, respectively. The substrate hydrolysis is monitored by measuring absorbance at 405 nm at 25°C for up to 30 min using a SpectraMax 384 Plus plate reader and SoftMax. FXa activity for each substrate and inhibitor concentration pair is determined in duplicate. The Ki values are calculated by non-linear least-squares fitting of the steady-state substrate hydrolysis rates to the equation for competitive inhibition (Equation 1) using GRAFIT, where v equals reactions velocity in OD min?1, Vmax equals maxiumum reaction velocity, S equals substrate concentration, and I equals inhibitor concentration. |
Cell Research | Genistein is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. The IC50 values for Genistein are determined by the MTT assay. Briefly, the MTT assay is a colorimetric assay that is based on the ability of living but not dead cells to reduce a tetrazolium-based compound to a blue formazan product. The formazan crystals are solubilized in DMSO, and the absorbance is measured at 540 nm. The absorbance at 540 nm is proportional to the number of viable cells. The lC50 values obtained with the MTT assay are compared with the lC50 values obtained by counting viable cells using trypan blue dye exclusion and by tritiated thymidine incorporation into DNA[1]. |
Source |
Synonyms | NPI 031L |
Molecular Weight | 270.24 |
Formula | C15H10O5 |
CAS No. | 446-72-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 54 mg/mL (199.82 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 2 mg/mL (7.4 mM)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Genistein 446-72-0 Angiogenesis Apoptosis Autophagy JAK/STAT signaling Metabolism Tyrosine Kinase/Adaptors Endogenous Metabolite EGFR NPI 031L HER1 ErbB-1 Inhibitor Epidermal growth factor receptor inhibit inhibitor