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Genistein

Catalog No. T1737   CAS 446-72-0
Synonyms: NPI 031L

Genistein (NPI 031L) is an isoflavonoid derived from soy products. It inhibits protein-tyrosine kinase and topoisomerase-II (DNA TOPOISOMERASES, TYPE II), with antineoplastic and antitumor activities.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Genistein Chemical Structure
Genistein, CAS 446-72-0
Pack Size Availability Price/USD Quantity
50 mg In stock $ 38.00
100 mg In stock $ 54.00
500 mg In stock $ 96.00
1 mL * 10 mM (in DMSO) In stock $ 57.00
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Purity: 99.69%
Purity: 98.22%
Purity: 98.22%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Genistein (NPI 031L) is an isoflavonoid derived from soy products. It inhibits protein-tyrosine kinase and topoisomerase-II (DNA TOPOISOMERASES, TYPE II), with antineoplastic and antitumor activities.
Targets&IC50 EGFR:0.6 μM (IC50, Cell Assay)
Kinase Assay Purified FXa is obtained after activation with Russell's viper venom followed by affinity chromatography. The resulting FXa is > 95% pure as judged by sodium dodecylsulfate polyacrylamide gel electrophoresis. The substrate affinity values for FXa, expressed as the Michaelis-Menten-Henri constant (Km), for human, rabbit, rat and dog FXa are determined using the chromogenic substrate S-2765, and are 36, 60, 240 and 70 μM, respectively. The substrate hydrolysis is monitored by measuring absorbance at 405 nm at 25°C for up to 30 min using a SpectraMax 384 Plus plate reader and SoftMax. FXa activity for each substrate and inhibitor concentration pair is determined in duplicate. The Ki values are calculated by non-linear least-squares fitting of the steady-state substrate hydrolysis rates to the equation for competitive inhibition (Equation 1) using GRAFIT, where v equals reactions velocity in OD min?1, Vmax equals maxiumum reaction velocity, S equals substrate concentration, and I equals inhibitor concentration.
Cell Research Genistein is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. The IC50 values for Genistein are determined by the MTT assay. Briefly, the MTT assay is a colorimetric assay that is based on the ability of living but not dead cells to reduce a tetrazolium-based compound to a blue formazan product. The formazan crystals are solubilized in DMSO, and the absorbance is measured at 540 nm. The absorbance at 540 nm is proportional to the number of viable cells. The lC50 values obtained with the MTT assay are compared with the lC50 values obtained by counting viable cells using trypan blue dye exclusion and by tritiated thymidine incorporation into DNA[1].
Source
Synonyms NPI 031L
Molecular Weight 270.24
Formula C15H10O5
CAS No. 446-72-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 54 mg/mL (199.82 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 2 mg/mL (7.4 mM)

TargetMolReferences and Literature

1. Akiyama T, et al. J Biol Chem, 1987, 262(12), 5592-5595. 2. Linassier C, et al. Biochem Pharmacol, 1990, 39(1), 187-193. 3. Dang ZC, et al. J Biol Chem, 2003, 278(2), 962-967. 4. Lamartiniere CA, et al. J Nutr, 2002, 132(3), 552S-558S. 5. Wang Y, et al. Radiat Res, 2006, 166(1 Pt 1), 73-80. 6. Jalili C, et al. Effect of Genistein on reproductive parameter and serum nitric oxide levels in morphine-treated mice. Int J Reprod Biomed (Yazd). 2016 Feb;14(2):95-102. 7. Ding S, et al. Environmentally Relevant Dose of Bisphenol A Does Not Affect Lipid Metabolism and Has No Synergetic or Antagonistic Effects on Genistein's Beneficial Roles on Lipid Metabolism. PLoS One. 2016 May 12;11(5):e0155352. 8. Zhou H, Ning Y, Zeng G, et al. Curcumin promotes cell cycle arrest and apoptosis of acute myeloid leukemia cells by inactivating AKT[J]. Oncology Reports. 2021, 45(4): 1-1.

TargetMolCitations

1. Ferreira R S B, Farias F O, de Araujo E J S, et al.Deep eutectic solvents as an alternative for extraction of flavonoids from soybean (Glycine max (L) Merrill) and okara: An experimental and computational approach based on COSMO-SAC model.Food Research International.2023: 113266. 2. Qin H, Song Z, Zhao C, et al.miR-363-3p/PTEN is involved in the regulation of lipid metabolism by genistein in HepG2 cells via ERβ.Phytomedicine.2023: 154839. 3. Cui L, Xu Q, Zhang P, et al.Biochemical reprogramming of active-targeting and redox responsive nanoparticles for enhancing selective internalization.Materials Letters.2023: 134379. 4. Zhang X, Hong K, Sun Q, et al. Bioreducible, arginine-rich polydisulfide-based siRNA nanocomplexes with excellent tumor penetration for efficient gene silencing. Biomaterials Science. 2021, 9(15): 5275-5292. 5. Zhou H, Ning Y, Zeng G, et al. Curcumin promotes cell cycle arrest and apoptosis of acute myeloid leukemia cells by inactivating AKT. Oncology Reports. 2021, 45(4): 1-1.

Related compound libraries

This product is contained In the following compound libraries:
Antiparasitic Natural Product Library Anti-Cancer Clinical Compound Library Anti-Cancer Active Compound Library Miao medicine Compound Library Anti-Cancer Drug Library Tyrosine Kinase Inhibitor Library Tobacco Monomer Library Anti-Cancer Approved Drug Library Anti-Colorectal Cancer Traditional Chinese Medicine Compound Library CNS-Penetrant Compound Library

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Keywords

Genistein 446-72-0 Angiogenesis Apoptosis Autophagy JAK/STAT signaling Metabolism Tyrosine Kinase/Adaptors Endogenous Metabolite EGFR NPI 031L HER1 ErbB-1 Inhibitor Epidermal growth factor receptor inhibit inhibitor

 

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