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ITE

Catalog No. T7202 CAS 448906-42-1

ITE is a potent endogenous agonist of aryl hydrocarbon receptor (AhR) (Ki : 3 nM), has immunosuppressive activity.

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ITE, CAS 448906-42-1

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Purity: 98%

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Description	ITE is a potent endogenous agonist of aryl hydrocarbon receptor (AhR) (Ki : 3 nM), has immunosuppressive activity.
Targets&IC50	AhR:3 nM (Ki)
In vitro	ITE dose- and time-dependently inhibited proliferation of HPAECs with a maximum inhibition of 83% at 20 μM after 6 days of treatment. ITE rapidly decreased AhR protein levels, while it increased mRNA levels of cytochrome P450 (CYP), family 1, member A1 (CYP1A1) and B1 (CYP1B1), indicating activation of the AhR/CYP1A1 and AhR/CYP1B1 pathways in HPAECs. The AhR siRNA significantly suppressed AhR protein expression, whereas it did not significantly alter ITE-inhibited growth of HPAECs[1].
In vivo	ITE diminishes colitis pathology through induction of Tregs; reduces inflammatory cytokines, inflammation score, and macrophage frequency; and induces DCs resulting in amelioration of colitis. Therefore, nontoxic endogenous ITE promotes the induction of Tregs and may be useful for the treatment of IBD[2].
Cell Research	Immunohistochemistry was performed to localize AhR expression in human lung tissues. The crystal violet method and MTT assay were used to determine ITE's effects on growth of HPAECs. The AhR activation in HPAECs was confirmed using Western blotting and RT-qPCR. The role of AhR in ITE-affected proliferation of HPAECs was assessed using siRNA knockdown method followed by the crystal violet method[1]
Animal Research	At the start of DSS induction, mice received 100 μl by intraperitoneal injection of vehicle and ITE (10 mg/kg body wt) twice a week on each Monday and Thursday until week 6 at the end point of the experiment. During a pilot study, we used several (5, 10, 20, 40, and 80 mg/kg body wt) doses of ITE and noticed that the 10-mg/kg dose was the lowest dose giving maximum protection. Therefore, Used this dose in entire study. At the experimental end point blood was collected by tail-vein bleedings and serum was obtained following centrifugation. For comparison, a similar treatment was also given to normal BL/6 mice to see the effect of ITE alone[2].

Molecular Weight	286.31
Formula	C14H10N2O3S
CAS No.	448906-42-1

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 41 mg/mL (143.20 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Pang L P , Li Y , Zou Q Y , et al. ITE inhibits growth of human pulmonary artery endothelial cells[J]. Experimental Lung Research, 2017, 43(8):283-292. 2. Abron J D , Singh N P , Mishra M K , et al. An endogenous aryl hydrocarbon receptor (AhR) ligand, ITE induces regulatory T cells (Tregs) and ameliorates experimental colitis[J]. American Journal of Physiology-Gastrointestinal and Liver Physiology, 2018:ajpgi.00413.2017-.

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Keywords

ITE 448906-42-1 Immunology/Inflammation Metabolism Aryl Hydrocarbon Receptor AhR Inhibitor inhibit inhibitor

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