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LM22B-10

Catalog No. T4496 CAS 342777-54-2

Synonyms: LM22B10

LM22B-10 is an activator of TrkB/TrkC neurotrophin receptor and can induce TrkB, TrkC, ERK and AKT activation in vitro and in vivo.

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LM22B-10, CAS 342777-54-2

Pack Size	Availability	Price/USD
1 mg	In stock	$ 54.00
2 mg	In stock	$ 84.00
5 mg	In stock	$ 128.00
10 mg	In stock	$ 216.00
25 mg	In stock	$ 497.00
50 mg	In stock	$ 747.00
100 mg	In stock	$ 1,050.00
1 mL * 10 mM (in DMSO)	In stock	$ 135.00

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Purity: 98.19%

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Description	LM22B-10 is an activator of TrkB/TrkC neurotrophin receptor and can induce TrkB, TrkC, ERK and AKT activation in vitro and in vivo.
In vitro	LM22B-10 exhibits superior neurotrophic activity, surpassing the highest levels of neurotrophic survival activity achieved by BDNF (53 ± 7.2% above BDNF at 0.7 nM) and NT-3 (91 ± 8.6% above NT-3 at 0.7 nM), with an EC50 value between 200-300 nM. At a concentration of 1000 nM, LM22B-10 significantly increases neurite length, achieving an average increment up to -40 μM. Furthermore, LM22B-10 demonstrates dose-dependent binding to TrkB-Fc and TrkC-Fc across a concentration range of 250-2000 nM. It effectively inhibits the binding of BDNF to TrkB-expressing cells and NT-3 to TrkC-expressing cells, supporting cell survival and preferentially engaging TrkB and TrkC pathways. Notably, LM22B-10 promotes neurite outgrowth even in inhibitory conditions, a capability not displayed by BDNF or NT-3. It triggers distinct Trk and subsequent signaling activation patterns, as well as stimulating TrkB, TrkC, AKT, and ERK activation in cultured hippocampal neurons, establishing its unique and potent neurotrophic effects compared to those of BDNF and NT-3[1].
In vivo	LM22B-10, at a dosage of 0.5 mg/kg, stimulates TrkB, TrkC, AKT, and ERK pathways in C57BL/6J mice. At a higher concentration of 50 mg/kg administered intraperitoneally (i.p.), it induces enhanced phosphorylation at TrkBY817 and TrkCY820. Furthermore, LM22B-10 triggers synaptic activation of TrkB and TrkC, elevating both pre- and post-synaptic protein levels and augmenting spine density in elderly mice [1].
Cell Research	Mouse NIH-3T3 cells, mouse NIH-3T3 cells expressing TrkA (NIH-3T3-TrkA) or p75NTR (NIH-3T3-p75NTR), and NIH-3T3 cells expressing TrkB (NIH-3T3-TrkB) or TrkC (NIH-3T3-TrkC) are propagated in DMEM supplemented with 10% FBS and 200-400 μg/mL Geneticin (for Trk-expressing cells) or 400 μg/mL hygromycin (for p75NTR-expressing cells). Cells are seeded into 24-well plates (30,000 cells/well) and cultured in medium consisting of 50% PBS and 50% DMEM without supplements. Following exposure to growth factors (0.7 nM) or 1000 nM LM22B-10 for 72-96 h, cells are suspended in 50 μL lysis buffer, transferred to opaque 96-well culture plates and survival is measured using the ViaLight Assay.

Synonyms	LM22B10
Molecular Weight	485.01
Formula	C27H33ClN2O4
CAS No.	342777-54-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 4.5 mg/mL

DMSO: 50 mg/mL (103.09 mM)

References and Literature

1. Yang T, et al. A small molecule TrkB/TrkC neurotrophin receptor co-activator with distinctive effects on neuronal survival and process outgrowth. Neuropharmacology. 2016 Nov;110(Pt A):343-61.

Related compound libraries

This product is contained In the following compound libraries：

Tyrosine Kinase Inhibitor Library Kinase Inhibitor Library Bioactive Compound Library Anti-Breast Cancer Compound Library Angiogenesis related Compound Library PI3K-AKT-mTOR Compound Library Glycolysis Compound Library Anti-Obesity Compound Library Oxidation-Reduction Compound Library Cytoskeletal Signaling Pathway Compound Library

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Keywords

LM22B-10 342777-54-2 Cytoskeletal Signaling MAPK PI3K/Akt/mTOR signaling Tyrosine Kinase/Adaptors ERK Akt Trk receptor inhibit Trk Receptor LM-22B-10 LM22B 10 LM22B10 Tropomyosin related kinase receptor PKB Inhibitor Protein kinase B Extracellular signal regulated kinases inhibitor

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