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TBHQ

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Purity:98%

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COA HNMR

TBHQ

Catalog No. T5364Cas No. 1948-33-0

TBHQ (tert-Butylhydroquinone) is an antioxidant that induces an antioxidant response through the redox-sensitive transcription factor Nrf2.

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Pack Size	Price	Availability
500 mg	$41	In Stock
1 g	$48	In Stock
1 mL x 10 mM (in DMSO)	$45	In Stock

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Product Introduction

Bioactivity

Description	TBHQ (tert-Butylhydroquinone) is an antioxidant that induces an antioxidant response through the redox-sensitive transcription factor Nrf2.
In vitro	HeLa cells treated with tBHQ showed preferential oxidation of mitochondrial thioredoxin-2 (Trx2), while cellular glutathione and cytosolic thioredoxin-1 were not affected. With tBHQ treatments, Trx2 overexpression suppressed Nrf2 accumulation and activity [1]. Pretreatment of Jurkat T cells with tBHQ, prior to activation with anti-CD3/anti-CD28, diminished the production of interleukin-2 (IL-2) at both the transcript and protein levels. Similarly, the expression of CD25 also diminished, albeit to a lesser degree than IL-2, after pretreatment with tBHQ [2].
In vivo	TBHQ treatment prevented left ventricular dilatation and cardiac dysfunction induced by TAC and decreased the prevalence of myocardial apoptosis. TBHQ-induced Akt activation was accompanied by increased phosphorylation of Bad, glycogen synthase kinase-3β (GSK-3β) and mammalian target of rapamycin (mTOR). Blockade of the Akt pathway in vivo accelerated cardiac dysfunction, and abrogated the protective effects of TBHQ. TBHQ also reduced the reactive aldehyde production and protein carbonylation in stressed myocardium [3].
Cell Research	Jurkat T cells were cultured in 96-well plates (1×10^5 cells/well) and treated as described, and cell supernatants were harvested 24h after activation with anti-CD3/anti-CD28. IL-2 was quantified from cell supernatants by the use of a commercially available kit per the manufacturer's protocol. Relative light intensity was quantified at 450nm by Bio-Tek μQuant microplate reader. The IL-2 concentrations of each sample were then calculated using a line ar standard curve on Microsoft Excel [2].
Animal Research	TBHQ powder was directly mixed with the animal food powder at a ratio of 1% (w/w) as described previously. The efficacy of this protocol for TBHQ treatment in vivo has also been validated by other groups. TBHQ treatment was continued for 4 weeks after the TAC surgery [3].
Alias	tert-Butylhydroquinone

Chemical Properties

Molecular Weight	166.22
Formula	C10H14O2
Cas No.	1948-33-0

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 50 mg/mL (300.81 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	6.0161 mL	30.0806 mL	60.1612 mL	300.8062 mL
5 mM	1.2032 mL	6.0161 mL	12.0322 mL	60.1612 mL
10 mM	0.6016 mL	3.0081 mL	6.0161 mL	30.0806 mL
20 mM	0.3008 mL	1.5040 mL	3.0081 mL	15.0403 mL
50 mM	0.1203 mL	0.6016 mL	1.2032 mL	6.0161 mL
100 mM	0.0602 mL	0.3008 mL	0.6016 mL	3.0081 mL

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