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N6-methyladenosine

Catalog No. T6599 CAS 1867-73-8

Synonyms: N-Methyladenosine, 6-Methyladenosine, NSC-29409, m6A

N6-methyladenosine (NSC-29409) is a methylated adenine residue. N6-Methyladenosine is an endogenous urinary nucleoside product of the degradation of transfer ribonucleic acid (tRNA); urinary nucleosides are biological markers for patients with colorectal cancer.

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N6-methyladenosine, CAS 1867-73-8

Pack Size	Availability	Price/USD
50 mg	In stock	$ 41.00
100 mg	In stock	$ 66.00
200 mg	In stock	$ 96.00
500 mg	In stock	$ 161.00
1 mL * 10 mM (in DMSO)	In stock	$ 45.00

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Purity: 99.66%

Purity: 98.63%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	N6-methyladenosine (NSC-29409) is a methylated adenine residue. N6-Methyladenosine is an endogenous urinary nucleoside product of the degradation of transfer ribonucleic acid (tRNA); urinary nucleosides are biological markers for patients with colorectal cancer.
In vitro	N6-Methyladenosine is an abundant modification in mRNA and is found within some viruses, and most eukaryotes including mammals, insects, plants and yeast. It is also found in tRNA, rRNA, and small nuclear RNA(snRNA) as well as several long non-coding RNA, such as Xist. [1] [2] [3] N6-Methyladenosine is an endogenous urinary nucleoside product of the degradation of transfer ribonucleic acid (tRNA). [4] N6-Methyladenosine is a widespread RNA modification in many tissues with high levels in the brain. N6-Methyladenosine is enriched near stop codons and within 3'UTRs in both mouse and human mRNAs. [5] The recent discovery that FTO, an obesity risk gene, encodes an m6A demethylase implicates m6A as an important regulator of physiological processes. [6]
In vivo	LD50: Mice >1 g/kg (i.g.). [7]
Kinase Assay	NOD1 Dose Response assay: Day 1 Procedure 1) Harvest HEK-293-T NFKB-Luc at 100% confluency at 100% confluency. 2) Add NOD assay media with Multidrop. 3) Spin down plates at 1000 rpm for 1 min in centrifuge. 4) Serial compound dilutions. 5) Add gamma-tri-DAP to cell suspension at 0.75 ug/mL. 6) Seed 13000 cells/well in 4 uL/well to full plate HEK-293-T NFKB-Luc to TC-treated plate. 7) Spin down plates 500 RPM for 5 min on centrifuge. 8) Lid Plates. Sandwich 4 plates between 2 lidded 384 plates filled with Water. 9) Wrap plates securely in single layer of Plastic Wrap. 10) Incubate overnight (14 hours) in 37 ℃ and 5% CO2 incubator. Day 2 Procedure 1) Add 3 ul/well of SteadyGlo solution with Multidrop. 2) Shake plates on a plate shaker for 20 min. 3) Spin plates 1000 RPM for 1 min using centrifuge. 4) Read luminescence.IC50 values are calculated using GraphPad Prism 5.0.The average Z' for the screen is 0.6, the signal to background is 11.1, signal to noise is 78.6 and signal to window is 6.0.

Synonyms	N-Methyladenosine, 6-Methyladenosine, NSC-29409, m6A
Molecular Weight	281.27
Formula	C11H15N5O4
CAS No.	1867-73-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 2 mg/mL (7.11 mM)

DMSO: 56 mg/mL (199.09 mM)

H2O: 14 mg/mL (49.77 mM)

References and Literature

1. Aloni Y, et al. J Virol, 1979, 32(1), 52-60. 2. Desrosiers R, et al. Proc Natl Acad Sci, 1974, 71(10), 3971-3975. 3. Adams JM, et al. Nature, 1975, 255(5503), 28-33. 4. Zheng YF, et al. World J Gastroenterol, 2005, 11(25), 3871-3876. 5. Meyer KD, et al. Cell, 2012, 149(7), 1635-1646. 6. Jia G, et al. Nat Chem Biol, 2011, 7(12), 885-887. 7. Li Y, et al. Genome-wide detection of high abundance N6-methyladenosine sites by microarray. RNA. 2015 Aug;21(8):1511-8. 8. Zhou L, Tang R, Li X, et al. N6-methyladenosine RNA modification regulates strawberry fruit ripening in an ABA-dependent manner[J]. bioRxiv. 2021

Citations

1. Zhou L, Tian S, Qin G. RNA methylomes reveal the m6A-mediated regulation of DNA demethylase gene SlDML2 in tomato fruit ripening. genome biology. 2019, 20(1): 1-23. 2. Zhou L, Tang R, Li X, et al. N6-methyladenosine RNA modification regulates strawberry fruit ripening in an ABA-dependent manner. Genome Biology. 2021, 22(1): 1-32 3. Li B, Zhang M, Sun W, et al.N6‐methyladenosine RNA modification regulates cotton drought response in a Ca2+ and ABA‐dependent manner.Plant Biotechnology Journal.2023 4. Li H, Yu K, Hu H, et al.METTL17 coordinates ferroptosis and tumorigenesis by regulating mitochondrial translation in colorectal cancer.Redox Biology.2024: 103087.

Related compound libraries

This product is contained In the following compound libraries：

Human Endogenous Metabolite Library Human Endogenous Metabolite Compound Library Plus Anti-Tumor Natural Product Library Natural Product Library for HTS Nucleotide Compound Library Human Metabolite Library Natural Product Library Anti-COVID-19 Compound Library RO5 Drug-like Natural Product Library Gut Microbial Metabolite Library

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Keywords

N6-methyladenosine 1867-73-8 Metabolism Microbiology/Virology Others Influenza Virus Endogenous Metabolite N-6-methyladenosine inhibit N6-Methyladenosine Inhibitor N-Methyladenosine 6-Methyladenosine N6methyladenosine N6 methyladenosine NSC-29409 m6A NSC 29409 NSC29409 inhibitor

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