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Protopine

Catalog No. T3429 CAS 130-86-9

Synonyms: Protopine, Reactive Oxygen Species, Corydinine, Biflorine, Inhibitor, Macleyine, inhibit, Fumarine, Hypercorine

Protopine is a benzylisoquinoline alkaloid. It has been found to inhibit histamine H1 receptors and platelet aggregation, and acts as an analgesic.

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Protopine, CAS 130-86-9

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Purity: 98%

Biological Description

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Storage & Solubility Information

Description

Protopine is a benzylisoquinoline alkaloid. It has been found to inhibit histamine H1 receptors and platelet aggregation, and acts as an analgesic.

Kinase Assay

HT1080 cells are seeded in 24-well plates (2×104 cells per well) and treated with Dorsomorphin in the presence or absence of glucose or 10 mM 2DG for 2 h. HT1080 cells that overexpressed the wild-type and dominant negative AMPKα1 are prepared by transfecting plasmid DNA (pAMPKα1-wt, pAMPKα1-D168A and pcFlag as a control) in 6-well plates, seeding in 24-well plate and treating with UPR inhibitors. Cells are lysed with cell lysis buffer (20 mM Tris-HCl, pH 7.5, 250 mM NaCl, 10% glycerol, 0.5% NP-40, 1 mM EDTA, 1 mM EGTA, 0.2 mM PMSF, 1 μg/mL pepstatin, 0.5 μg/mL leupeptin, 5 mM NaF, 2 mM Na3Vo4, 2 mM β-glycerophosphate, 1 mM DTT). Relative AMPK kinase activity (mean±SD of duplicate determinations) to control sample (vehicle or pcFlag under normal growth conditions) is determined using the CycLex AMPK kinase assay kit[2].

Synonyms	Protopine, Reactive Oxygen Species, Corydinine, Biflorine, Inhibitor, Macleyine, inhibit, Fumarine, Hypercorine
Molecular Weight	353.374
Formula	C20H19NO5
CAS No.	130-86-9

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 28 mg/mL(79.2 mM)

Chloroform 1:15: Soluble

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature

1. Saeed SA, et al. Pharmacol Res. 1997 Jul;36(1):1-7.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Terpene Natural Product Library GPCR Compound Library Traditional Chinese Medicine Monomer Library Tibetan medicine Compound Library Coagulation and Anticoagulation Compound Library Histamine & Melatonin Receptor-Targeted Compound Library Neurotransmitter Receptor Compound Library Inhibitor Library Natural Product Library for HTS

Related Products

Related compounds with same targets

Zeorin Corchoionoside C 3,5-Dicaffeoyl-epi-quinic acid Marmin (-)-Maackiain Cyclizine dihydrochloride Cetirizine D8 dihydrochloride Toreforant

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