Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Protopine (Fumarine) is a benzylisoquinoline alkaloid. It has been found to inhibit histamine H1 receptors and platelet aggregation, and acts as an analgesic.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
20 mg | In stock | $ 50.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 50.00 |
Description | Protopine (Fumarine) is a benzylisoquinoline alkaloid. It has been found to inhibit histamine H1 receptors and platelet aggregation, and acts as an analgesic. |
Kinase Assay | HT1080 cells are seeded in 24-well plates (2×104 cells per well) and treated with Dorsomorphin in the presence or absence of glucose or 10 mM 2DG for 2 h. HT1080 cells that overexpressed the wild-type and dominant negative AMPKα1 are prepared by transfecting plasmid DNA (pAMPKα1-wt, pAMPKα1-D168A and pcFlag as a control) in 6-well plates, seeding in 24-well plate and treating with UPR inhibitors. Cells are lysed with cell lysis buffer (20 mM Tris-HCl, pH 7.5, 250 mM NaCl, 10% glycerol, 0.5% NP-40, 1 mM EDTA, 1 mM EGTA, 0.2 mM PMSF, 1 μg/mL pepstatin, 0.5 μg/mL leupeptin, 5 mM NaF, 2 mM Na3Vo4, 2 mM β-glycerophosphate, 1 mM DTT). Relative AMPK kinase activity (mean±SD of duplicate determinations) to control sample (vehicle or pcFlag under normal growth conditions) is determined using the CycLex AMPK kinase assay kit[2]. |
Source |
Synonyms | Macleyine, Hypercorine, Corydinine, Biflorine, Fumarine |
Molecular Weight | 353.37 |
Formula | C20H19NO5 |
CAS No. | 130-86-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 28 mg/mL(79.2 mM)
Chloroform 1:15: Soluble
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Protopine 130-86-9 GPCR/G Protein Immunology/Inflammation Metabolism Neuroscience NF-Κb Others Platelet aggregation Histamine Receptor Reactive Oxygen Species Macleyine Hypercorine Inhibitor Corydinine inhibit Biflorine Fumarine inhibitor