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VCMMAE

🥰Excellent
Catalog No. T4232Cas No. 646502-53-6
Alias mc-vc-PAB-MMAE

VCMMAE (mc-vc-PAB-MMAE) is a drug-linker conjugate for ADC with potent antitumor activity.

VCMMAE

VCMMAE

🥰Excellent
Purity: 100%
Catalog No. T4232Alias mc-vc-PAB-MMAECas No. 646502-53-6
VCMMAE (mc-vc-PAB-MMAE) is a drug-linker conjugate for ADC with potent antitumor activity.
Pack SizePriceAvailabilityQuantity
2 mg$30In Stock
5 mg$48In Stock
10 mg$89In Stock
25 mg$163In Stock
50 mg$263In Stock
100 mg$397In Stock
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Questions
QDo inhibitors need to be sterilized?
AIf you are using DMSO for preparation,sterilization is not recommended, as DMSO itself possesses strong sterilizing properties. The prepared solution is sterile. For further assurance, it can be left overnight at 4°C. The safest method is to filter through a specialized organic filter membrane. If water is used for preparation,sterilization can be achieved by filtering through a 0.22 μm filter membrane.
QWhat should I do if the IC50 obtained for cell killing are significantly different from that presented on the your website?
AThe IC50 you obtain refers to the cell proliferation inhibition assay (half maximal inhibitory concentration), which is different in meaning from the IC50 on our website. The IC50 often refers to the inhibition rate of the target without cell experiments, which is generally determined through kinase or protein purification experiments, and is commonly reported in nanomolar concentrations. However, the IC50 obtained from cell proliferation inhibition assays refers to the half-maximal lethality of the cells, which involves cellular metabolism and penetration, so the concentration is usually higher. Furthermore, the effect of the same compound on different cell models varies. It is recommended to try increasing the incubation volume and extending the incubation time.
Qwhat frequency should sonication be used?
AIt is recommended to use the conventional ultrasound cleaning frequency
QCan the inhibitor DMSO stock solution be directly diluted with buffer to create a gradient?
AIn most cases, it can dissolve. However, sometimes organic reagents may precipitate when directly added to an aqueous medium. It is recommended to first dilute the inhibitor with DMSO to form a gradient, and then add the diluted inhibitor to the buffer or cell culture medium. Some inhibitors may only dissolve in the aqueous phase at their working concentrations. For example, if a final concentration of 1 μM is desired in cell experiments, the 10 mM DMSO stock solution can be diluted to 1 mM with DMSO, and then 2 μL can be drawn and added to 2 mL of saline/PBS/cell culture medium, resulting in a final concentration of 1 μM. To avoid precipitation of the drug, it is recommended to preheat the mother liquor and culture medium to 37°C before dilution to avoid serious precipitation caused by low temperature. If precipitation occurs during the dilution process, it is suggested to use ultrasonic heating to redissolve the compound.
QWhat are the common methods for dissolving compounds in animal experiments?
AFirst, you need to confirm the dosage and administration route. For specific products, it is recommended to refer to literatures that cite our product. If there are no relevant references and the compound dissolves well in DMSO, we recommend a general formula: 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline/PBS/ddH2O. The solvents should be added sequentially, and each solvent should be added after ensuring as much dissolution as possible. For normal mice, keep the DMSO concentration below 10%, and for nude mice and mice with weak tolerance, the DMSO concentration should be kept below 2%. The ratio of PEG300 and Tween-80 can be adjusted accordingly based on whether the solution is clear or not. Other co-solvents can also be used. The above formula is for reference only. Please adjust according to different conditions. It is recommended to first test a small amount of compound with the formula before preparing a large quantity. Additionally, sonication or heating can be used to accelerate dissolution. For intraperitoneal injection, it is preferable to use salt forms of compounds as they have higher solubility. If the dosage is large, suspension may be used for intraperitoneal administration. For oral gavage, especially with larger doses, it is recommended to prepare a homogeneous suspension using 0.5% CMC-Na.
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Purity:100%
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Product Introduction

Bioactivity
Description
VCMMAE (mc-vc-PAB-MMAE) is a drug-linker conjugate for ADC with potent antitumor activity.
In vitro
MMAE sensitized colorectal and pancreatic cancer cells to IR in a schedule and dose dependent manner correlating with mitotic arrest. Monomethyl auristatin E (MMAE) is efficiently released from SGN-35 within CD30+ cancer cells and, owing to its membrane permeability, it has the ability to exert cytotoxic activity on bystander cells. Radiosensitization is evidenced by decreased clonogenic survival and increased DNA double strand breaks in irradiated cells.
In vivo
Monomethyl auristatin E (MMAE), when combined with IR, enhances DNA damage signaling and CHK1 activation, delaying tumor growth. Tumor-targeted ACPP-cRGD-MMAE with IR induces more robust and significantly prolonged tumor regression in xenograft models.
Cell Research
Monomethyl auristatin E is reconstituted in DMSO at a concentration of 5 nM. Monomethyl auristatin E (MMAE, 5 nM) and ionizing radiation (IR) treated cells are harvested and lysed in RIPA buffer with protease and phosphatase inhibitors. 30 μg of lysate undergo electrophoresis using 4-12% Bis-Tris gels, transferred to PVDF membranes and incubated with indicated primary antibodies.
Animal Research
6-8 week old female athymic nu/nu mice are injected subcutaneously into thighs with 5×106 HCT-116 or PANC-1 cells in a 1:1 Matrigel and PBS solution. Mice are treated with IR or intravenous (IV) injection of ACPP-cRGD-MMAE (6 nmoles/day, 18 nmoles total, i.v.), tumor tissue is harvested, formalin fixed and paraffin embedded followed by staining with indicated antibodies. The primary antibody is used at a 1:250 dilution and is visualized using DAB as a chromagen with the UltraMap system.
Aliasmc-vc-PAB-MMAE
Chemical Properties
Molecular Weight1316.63
FormulaC68H105N11O15
Cas No.646502-53-6
SmilesCC[C@H](C)[C@@H]([C@@H](CC(=O)N1CCC[C@H]1[C@H](OC)[C@@H](C)C(=O)N[C@H](C)[C@@H](O)c1ccccc1)OC)N(C)C(=O)[C@@H](NC(=O)[C@H](C(C)C)N(C)C(=O)OCc1ccc(NC(=O)[C@H](CCCNC(N)=O)NC(=O)[C@@H](NC(=O)CCCCCN2C(=O)C=CC2=O)C(C)C)cc1)C(C)C
Relative Density.1.196 g/cm3 (Predicted)
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 54 mg/mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
1 Enter information below:
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2 Enter the in vivo formulation:
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References

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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