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AP 18

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Purity:99.85%

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COA HNMR HPLC

AP 18

Catalog No. T21543Cas No. 55224-94-7

AP-18 is a potent and selective TRPA1 inhibitor. AP-18 inhibits activation of TRPA1 induced by 50 μM Cinnamaldehyde with an IC50 of 3.1 μM and 4.5 μM for human and mouse TRPA1, respectively. AP-18 could reverse complete Freund's adjuvant (CFA)-induced mechanical hyperalgesia in mice. AP-18 could attenuate Yo-Pro uptake induced by 30 μM AITC in a concentration-dependent manner (IC50= 10.3 μM).

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Pack Size	Price	Availability
2 mg	$30	In Stock
5 mg	$43	In Stock
10 mg	$67	In Stock
25 mg	$143	In Stock
50 mg	$226	In Stock
100 mg	$355	In Stock
500 mg	$762	In Stock
1 mL x 10 mM (in DMSO)	$43	In Stock

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Product Introduction

Bioactivity

Description	AP-18 is a potent and selective TRPA1 inhibitor. AP-18 inhibits activation of TRPA1 induced by 50 μM Cinnamaldehyde with an IC50 of 3.1 μM and 4.5 μM for human and mouse TRPA1, respectively. AP-18 could reverse complete Freund's adjuvant (CFA)-induced mechanical hyperalgesia in mice. AP-18 could attenuate Yo-Pro uptake induced by 30 μM AITC in a concentration-dependent manner (IC50= 10.3 μM).
In vitro	At concentrations up to 50 μM, AP-18 does not significantly inhibit the activation of TRPV1, TRPV2, TRPV3, TRPV4, or TRPM8. However, it effectively and reversibly inhibits the activation of mouse TRPA1 by iodoacetamide (an irreversible cysteine-alkylating agent) in CHO cells, as determined through ratiometric Ca2+ imaging. Additionally, AP-18 prevents cold- and mustard-oil-triggered activation of mouse TRPA1 and inhibits TRPA1 currents induced by cinnamaldehyde in Xenopus oocyte excised patches, demonstrating its specificity and effectiveness in modulating TRPA1 activity [1] [2] [3].
In vivo	AP18 (1 mM; injected in the hindpaw of mice) effectively inhibits nociceptive events triggered by cinnamaldehyde but not those induced by capsaicin, evidencing its specificity and efficacy [1].

Chemical Properties

Molecular Weight	209.67
Formula	C11H12ClNO
Cas No.	55224-94-7

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 50 mg/mL (238.47 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.7694 mL	23.8470 mL	47.6940 mL	238.4700 mL
5 mM	0.9539 mL	4.7694 mL	9.5388 mL	47.6940 mL
10 mM	0.4769 mL	2.3847 mL	4.7694 mL	23.8470 mL
20 mM	0.2385 mL	1.1923 mL	2.3847 mL	11.9235 mL
50 mM	0.0954 mL	0.4769 mL	0.9539 mL	4.7694 mL
100 mM	0.0477 mL	0.2385 mL	0.4769 mL	2.3847 mL

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