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Product Introduction

Bioactivity

Description	Cilobradine hydrochloride (DK-AH 269) is an HCN Channel blocker and an open channel blocker of neuronal Ih and related cardiac If channels.
In vitro	Cilobradine hydrochloride is an HCN channel blocker that is about 3 times more potent than ZD7288. At a concentration of 10 μM, Cilobradine hydrochloride inhibits WT mHCN2 channel current by 86%. In contrast, I432A and A425G channel currents were only reduced by 14% and 19%, respectively, by this concentration of Cilobradine hydrochloride. The double mutant (I432A/A425G) channel was even less sensitive to 10 μM Cilobradine hydrochloride (8 ± 2% inhibition)[1].
Alias	DK-AH 269

Chemical Properties

Molecular Weight	519.07
Formula	C₂₈H₃₉ClN₂O₅
Cas No.	186097-54-1
Smiles	Cl.COc1ccc(CCN2CCC[C@H](CN3CCc4cc(OC)c(OC)cc4CC3=O)C2)cc1OC
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 112.5 mg/mL (216.7 mM)

H2O: 91 mg/mL (175.31 mM)

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9265 mL	9.6326 mL	19.2652 mL	96.3261 mL
5 mM	0.3853 mL	1.9265 mL	3.8530 mL	19.2652 mL
10 mM	0.1927 mL	0.9633 mL	1.9265 mL	9.6326 mL
20 mM	0.0963 mL	0.4816 mL	0.9633 mL	4.8163 mL
50 mM	0.0385 mL	0.1927 mL	0.3853 mL	1.9265 mL
100 mM	0.0193 mL	0.0963 mL	0.1927 mL	0.9633 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc