OAC1 (BAS 00287861) is a compound activating Octamer-binding transcription factor 4 (Oct4). It enhances the iPSC reprogramming efficiency and accelerates the reprogramming process.

OAC1 appears to enhance reprogramming efficiency by increasing transcription of the Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA demethylation. OAC1 does not inhibit the p53-p21 pathway or activate Wnt-β-catenin signaling.OAC1 can be used to enhance the reprogramming of somatic cells to a pluripotent state.1 μM OAC1 enhances the reprogramming efficiency by activating the Oct4 and Nanog promoter-driven luciferase reporter genes. In addition, OAC1 enhanced the reprogramming efficiency and accelerated the reprogramming process of pluripotent stem cells (iPSCs) in mouse embryonic fibroblasts (MEFs) treated with four reprogramming factors (Oct4, Sox2, c-Myc and Klf4).

Enzymology: Kinesin motor domains are expressed in Escherichia coli BL21(DE3) and purified. CENP-E proteins includes residues 2–340 with a carboxyl-terminal 6-his tag. All studies using MT are conducted in PEM25 buffer [25 mM PipesK+ (pH 6.8), 2 mM MgCl2, 1 mM EGTA] supplemented with 10 μM paclitaxel. The IC50 for steady-state inhibition is determined at 500 μM ATP, 5 μM MT, and 1 nM CENP-E in PEM25 buffer.

The Oct4-luc or Nanog-luc cells are treated with compound OAC1 at 1 μM concentration. Luciferase reporter assays are performed 24 h after OAC1 treatment.(Only for Reference)