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BAPTA-AM

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Purity:99.47%

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COA HPLC HNMR

BAPTA-AM

Catalog No. T6245Cas No. 126150-97-8

BAPTA-AM is a calcium chelator that is cell-permeable and selective, blocking hERG, hKv1.3, and hKv1.5 channels (IC50=1.3/1.45/1.23 μM). BAPTA-AM has a 105-fold higher affinity for Ca2+ than for Mg2+, and can be used for the role of calcium in cell signaling.

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Pack Size	Price	Availability
5 mg	$30	In Stock
10 mg	$51	In Stock
25 mg	Inquiry	In Stock
50 mg	Inquiry	In Stock
1 mL x 10 mM (in DMSO)	$43	In Stock

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Product Introduction

Bioactivity

Description	BAPTA-AM is a calcium chelator that is cell-permeable and selective, blocking hERG, hKv1.3, and hKv1.5 channels (IC50=1.3/1.45/1.23 μM). BAPTA-AM has a 105-fold higher affinity for Ca2+ than for Mg2+, and can be used for the role of calcium in cell signaling.
In vitro	METHODS: Chondrocytes were treated with BAPTA-AM (10 μM) and FAC (100 μM) for 24 h. Intracellular ROS levels were measured using the Reactive Oxygen Species Assay kit. RESULTS: FAC promoted ROS production and this effect was inhibited by the calcium chelator BAPTA-AM. [1] METHODS: Rat fibroblast RAT2 and Xenopus cells were treated with BAPTA-AM (50 μM) for 1 h, and microtubule depolymerization was detected by Immunostaining. RESULTS: BAPTA AM treatment for 30 min resulted in almost complete disassembly in most cells, and microtubules were uniformly depolymerized in cells within 60 min. [2]
In vivo	METHODS: To investigate the effect on ethanol-induced locomotor activity, BAPTA-AM (0-10 mg/kg, Cremophor EL 1.25% (v/v) in distilled water) was injected intraperitoneally into Swiss (RjOrl) mice, followed by ethanol (0-4 g/kg) 30 min later. RESULTS: Pretreatment with BAPTA-AM blocked the locomotor stimulus produced by ethanol without altering basal locomotion. On the contrary, BAPTA-AM reversed the ethanol-induced hypnosis. [3] METHODS: To investigate the effect on LPS-induced blood-brain barrier leakage, BAPTA-AM (12 mg/kg, 0.01% pluronic acid in sterile saline) was injected intravenously into FVB mice, followed 30 min later by intraperitoneal injection of LPS (25 mg/kg). RESULTS: BAPTA-AM reduced LPS-induced blood-brain barrier leakage. [4]
Alias	BAPTA/AM

Chemical Properties

Molecular Weight	764.68
Formula	C34H40N2O18
Cas No.	126150-97-8

Storage & Solubility Information

Storage

keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 50 mg/mL (65.39 mM)

5% DMSO+95% Saline: 7.25 mg/mL (9.48 mM, precipitation)

Solution Preparation Table

DMSO/5% DMSO+95% Saline

	1mg	5mg	10mg	50mg
1 mM	1.3077 mL	6.5387 mL	13.0774 mL	65.3868 mL
5 mM	0.2615 mL	1.3077 mL	2.6155 mL	13.0774 mL

DMSO

	1mg	5mg	10mg	50mg
10 mM	0.1308 mL	0.6539 mL	1.3077 mL	6.5387 mL
20 mM	0.0654 mL	0.3269 mL	0.6539 mL	3.2693 mL
50 mM	0.0262 mL	0.1308 mL	0.2615 mL	1.3077 mL
100 mM	0.0131 mL	0.0654 mL	0.1308 mL	0.6539 mL

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