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BMS 777607

Catalog No. T2699 CAS 1025720-94-8

Synonyms: BMS777607, BMS-777607, BMS 817378

BMS 777607 (BMS 817378) is a Met-related inhibitor for c-Met/Axl/Ron/Tyro3 (IC50: 3.9/1.1/1.8/4.3 nM). BMS-777607 has been investigated for the basic science of Malignant Solid Tumour.

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BMS 777607, CAS 1025720-94-8

Pack Size	Availability	Price/USD
1 mg	In stock	$ 32.00
2 mg	In stock	$ 45.00
5 mg	In stock	$ 72.00
10 mg	In stock	$ 126.00
25 mg	In stock	$ 229.00
50 mg	In stock	$ 378.00
100 mg	In stock	$ 562.00
1 mL * 10 mM (in DMSO)	In stock	$ 84.00

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Purity: 100%

Purity: 99.89%

Purity: 99.74%

Purity: 99.22%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	BMS 777607 (BMS 817378) is a Met-related inhibitor for c-Met/Axl/Ron/Tyro3 (IC50: 3.9/1.1/1.8/4.3 nM). BMS-777607 has been investigated for the basic science of Malignant Solid Tumour.
Targets&IC50	RON:1.8 nM, MET:3.9 nM, Tyro3:4.3 nM, Axl:1.1 nM
Kinase Assay	Met Kinase Assay: The kinase reaction consists of baculovirus expressed GST-Met, 3 μg of poly(Glu/Tyr), 0.12 μCi 33P γ-ATP, 1 μM ATP in 30 μL of kinase buffer (20 mM Tris-Cl, 5 mM MnCl2, 0.1 mg/mL BSA, 0.5 mM DTT). Reactions are incubated for 1 hour at 30 °C and stopped by the addition of cold trichloroacetic acid (TCA) to a final concentration of 8%. TCA precipitates are collected onto GF/C unifilter plates using a Filtermate universal harvester, and the filters are quantitated using a TopCount 96-well liquid scintillation counter. Dose response curves are generated to determine the concentration required to inhibit 50% of substrate phosphorylation (IC50). BMS 777607 is dissolved at 10 mM in dimethylsulfoxide (DMSO) and evaluated at 10 concentrations, in duplicate.
Cell Research	KHT cells are exposed to serial dilution of BMS 777607 for 96 hours, then the MTT assay and trypan blue exclusion are used for the determination of cell proliferation and cell death, respectively. KHT cell colonies are incubated with BMS 777607 for 24 hours and then stained with crystal violet (0.1%) and photographed for the assessment of cell scattering. 2 mm scratch on the confluent KHT cell monolayer is made using a sterilized 1 ml pipette tip followed by treated with BMS-777607 for 24 hours, then the number of cells that have migrated into the denuded area is counted on 4 random fields for the evaluation of cell migration. For the examination of cell invasion, the commercial transwell inserts (8 μm pore membrane) pre-loaded with Matrigel are incubated with serum-free medium in the presence or absence of BMS 777607 at 37 °C for 2 hours to allow rehydration of Matrigel. Then cells suspended in serum-free medium are loaded onto the top chamber (5 × 103/insert) and complete medium (containing 10% FBS) is used in the lower chamber as a chemoattractant. After incubation for 24 hours, the Matrigel is removed and the inserts are stained with crystal violet. Invaded cells on the underside of the filter are photographed and counted. (Only for Reference)

Synonyms	BMS777607, BMS-777607, BMS 817378
Molecular Weight	512.89
Formula	C25H19ClF2N4O4
CAS No.	1025720-94-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 44 mg/mL (85.8 mM)

References and Literature

1. Schroeder GM, et al. J Med Chem, 2009, 52(5), 1251-1254. 2. Dai Y, et al. Mol Cancer Ther, 2010, 9(6), 1554-1561. 3. Dai Y, et al. Clin Exp Metastasis, 2012, 29, 253-261. 4. Kim KS, et al. J Med Chem, 2008, 51(17), 5330-5341.

Related compound libraries

This product is contained In the following compound libraries：

Tyrosine Kinase Inhibitor Library Anti-Lung Cancer Compound Library Anti-Liver Cancer Compound Library ReFRAME Related Library NO PAINS Compound Library Reprogramming Compound Library Anti-Breast Cancer Compound Library Fluorochemical Library Anti-Colorectal Cancer Compound Library Hematopoietic Toxicity Compound Library

Related Products

Related compounds with same targets

PF-04217903 Antitumor agent-111 Ningetinib (±)-Norcantharidin Tepotinib hydrochloride(1 : x) c-Met-IN-2 JNJ-38877605 c-Met-IN-19

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

BMS 777607 1025720-94-8 Tyrosine Kinase/Adaptors TAM Receptor c-Met/HGFR Inhibitor Mer BMS777607 inhibit Axl Tyro3 BMS-817378 BMS817378 BMS-777607 BMS 817378 inhibitor

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