Home Tools

Cart

Bavachin

Catalog No. T3846 CAS 19879-32-4

Synonyms: Corylifolin

Bavachin (Corylifolin) is a phytoestrogen that activates the estrogen receptors ERα and ERβ.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Bavachin, CAS 19879-32-4

Pack Size	Availability	Price/USD
5 mg	In stock	$ 30.00
10 mg	In stock	$ 37.00
25 mg	In stock	$ 56.00
50 mg	In stock	$ 78.00
100 mg	In stock	$ 113.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

Bulk Inquiry

Select Batch

Purity: 99.91%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Bavachin (Corylifolin) is a phytoestrogen that activates the estrogen receptors ERα and ERβ.
Targets&IC50	ERβ:680 nM (EC50), ERα:320nM (EC50)
In vitro	Bavachin effectively reduces melanin production and tyrosinase (TYR) activity. At a concentration of 10 μM, it inhibits not only TYR and c-Jun N-terminal kinase (JNK) protein expression but also the expression of TYR, tyrosinase-related protein-1 (TRP-1), TRP-2, extracellular signal-regulated kinase 1 (ERK1), ERK2, and JNK2 mRNA in A375 cells. The effects of bavachin on protein and mRNA expression levels of TYR, TRP-1, TRP-2, ERK1, ERK2, and JNK2 are significantly reversed by ICI182780 and U0126. Furthermore, bavachin promotes lipid accumulation in a dose-dependent manner, evident in Oil Red O (ORO) staining experiments, and enhances preadipocyte growth at 10 μM, as demonstrated in MTT assay, compared to control cells. It also boosts bromodeoxyuridine (BrdU) incorporation into newly synthesized DNA during pre-adipocyte proliferation, a process further enhanced by insulin and by co-treatment with insulin and bavachin. Bavachin activates adipogenic factors, increases peroxisome proliferator-activated receptor gamma (PPARγ) transcriptional activity in differentiated adipocytes, and improves insulin-stimulated glucose uptake through glucose transporter type 4 (GLUT4) translocation via the Akt and AMP-activated protein kinase (AMPK) pathways. Additionally, bavachin significantly increases human monoamine oxidase-A (hMAO-A) and hMAO-B activities. It also exhibits estrogen receptor (ER) ligand-binding activity, displacing [3H] estradiol (E2) from recombinant ER, with its estrogenic activity characterized by half-maximal effective concentrations (EC50) of 320 nM for ERα and 680 nM for ERβ in a transient transfection system. Bavachin upregulates mRNA levels of estrogen-responsive genes such as pS2 and PR, while decreasing ERα protein level via the proteasomal pathway.
Kinase Assay	The chemiluminescent assay is used to confirm PCSEE MAO-A and MAO-B inhibitory effects and to test BNN and BVN hMAO-A and hMAO-B inhibition using MAO-Glo kit. Each enzyme's Arbitrary Light Unit (ALU) is measured in the presence of PCSEE, BNN, BVN, and standard DEP as an MAO-BI positive control. Briefly, hMAO-A and hMAO-B isozymes are diluted to 2× with reaction buffer (pH 7.4) and preincubated with 4× PCSEE, BNN, BVN, or DEP working solutions at RT for 30?min in white opaque 96-well plates. For determining activity inhibition, final 8.5?μg/mL concentrations of PCSEE, BNN, BVN, and DEP are used. For IC50 determination, 8× PCSEE and BNN working solutions are serially diluted using reaction buffers (pH 7.4) to make a 4× concentration. Ten points' range of PCSEE (1.0 to 250.0?μg/mL) and BNN (up to 400?μM (135.4?μg/mL)) final concentrations is used. Controls used are with and without ethanol. Ethanol solvent in controls is kept to a maximum final (volume) of ≤2%. Each isozyme is substituted with the reaction buffer for the blank. Based on our preliminary optimizations and Valley's method, the reaction is initiated by adding 4× luciferin derivative substrate (LDS) for a final (concentration) of 40 and 4?μM for hMAO-A and hMAO-B reactions, respectively. The final volume per well of each reaction is 50?μL. The reaction is optimized for the amount of A and B enzyme used to be incubated for less than 3.5?h at RT. To stop the reaction and produce the luminescence signal RLDR is added to all wells, 50?μL to each well, and incubated for a further 30?min.
Cell Research	Bavachin is dissolved in DMSO. MTT solution (20 μL) is added to each well of the 96-well plates, the cells are cultured for 4 h, the solution is discarded, and the purple crystal is dissolved in the wells with 150 μL DMSO solution, agitated in a 37°C incubator shaker for 10 min, and the optical density (OD) is measured at 490 nm by the microplate reader.
Source	Natural Products > Fabaceae > Psoralea

Synonyms	Corylifolin
Molecular Weight	324.37
Formula	C20H20O4
CAS No.	19879-32-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 58 mg/mL(178.8 mM)

Chloroform, Dichloromethane, Ethyl Acetate: Soluble

References and Literature

1. Wang JH, et al. Effects of bavachin and its regulation of melanin synthesis in A375 cells. Biomed Rep. 2016 Jul;5(1):87-92. Epub 2016 May 20. 2. Lee H, et al. Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes. Int J Mol Sci. 2016 Apr 8;17(4):527. 3. Zarmouh NO, et al. Evaluation of the Inhibitory Effects of Bavachinin and Bavachin on Human Monoamine Oxidases A and B. Evid Based Complement Alternat Med. 2015;22015:852194. 4. Park J, et al. Activation of Estrogen Receptor by Bavachin from Psoralea corylifolia. Biomol Ther (Seoul). 2012 Mar;20(2):183-8.

Citations

1. Luo Y, Gao X, Zou L, et al. Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells. Oxidative Medicine and Cellular Longevity. 2021

Related compound libraries

This product is contained In the following compound libraries：

Flavonoid Natural Product Library NO PAINS Compound Library Bioactive Compounds Library Max Selected Plant-Sourced Compound Library Traditional Chinese Medicine Monomer Library Anti-Tumor Natural Product Library Anti-Ovarian Cancer Compound Library RO5 Drug-like Natural Product Library Natural Product Library for HTS Natural Product Library

Related Products

Related compounds with same targets

Erteberel ER degrader 6 Endoxifen Clomiphene citrate (E/Z)-4-Hydroxytamoxifen Estrogen receptor modulator 10 G36 Estradiol dipropionate

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Bavachin 19879-32-4 Endocrinology/Hormones Estrogen Receptor/ERR Estrogen/progestogen Receptor Corylifolin Inhibitor inhibit inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.