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C527

Catalog No. T14852 CAS 192718-06-2

C527 is a is a pan DUB enzyme inhibitor. Which has a high potency for the USP1/UAF1 complex (IC50=0.88 μM).

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C527, CAS 192718-06-2

Pack Size	Availability	Price/USD
5 mg	In stock	$ 47.00
10 mg	In stock	$ 80.00
25 mg	In stock	$ 172.00
50 mg	In stock	$ 288.00
100 mg	In stock	$ 483.00

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Purity: 97.64%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	C527 is a is a pan DUB enzyme inhibitor. Which has a high potency for the USP1/UAF1 complex (IC50=0.88 μM).
Targets&IC50	USP1:0.88 μM
In vitro	C527 inhibits the DUB activity of the USP12/USP46 complex and other DUB enzymes in vitro. However, the IC50 of C527 for these DUB enzymes was higher in comparison with USP1/UAF1 complex. C527 has considerably less inhibitory effect on UCH-L1 and UCH-L3, a different subclass of DUB enzymes. C527 treatments causes an increase in the levels of Ub-FANCD2 and Ub-FANCI and it also lead to an increase in ubiquitinated forms of FANCD2 and FANCI, cause a decrease in homologous recombination activity, and sensitize cells to DNA damaging agents[1]. Pretreatment of USP1/UAF1 with C527 resulted in inhibition of its enzyme activity with an IC50 of 0.88±0.03 μM. Pretreatment of cells with the C527 causes an enhancement in the cytoxicity of mitomycin C and camptothecin.

Molecular Weight	293.25
Formula	C17H8FNO3
CAS No.	192718-06-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 1.85 mg/mL (6.31 mM), Sonification is recommended

References and Literature

1. Mistry H, et al. Small-molecule inhibitors of USP1 target ID1 degradation in leukemic cells. Mol Cancer Ther. 2013 Dec;12(12):2651-62.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Cell Cycle Compound Library HIF-1 Signaling Pathway Compound Library Bioactive Compound Library Ubiquitination Compound Library DNA Damage & Repair Compound Library Bioactive Compounds Library Max Anti-Cancer Compound Library

Related Products

Related compounds with same targets

GRL0617 GSK2643943A OTUB1/USP8-IN-1 IU1 P22074 LDN-91946 USP7/USP47 inhibitor DUB-IN-2

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

C527 192718-06-2 Cell Cycle/Checkpoint DNA Damage/DNA Repair Ubiquitination DUB Inhibitor DUBs C 527 C-527 Deubiquitinase inhibit inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.