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Hydrocortisone

Catalog No. T1614 CAS 50-23-7

Synonyms: Cortisol

Hydrocortisone (Cortisol) is a glucocorticoid hormone secreted by the adrenocortex. Hydrocortisone agonizes the glucocorticoid receptor, which promotes proteolytic metabolism, gluconeogenesis, capillary wall stabilization, and renal calcium excretion, as well as suppressing immune and inflammatory responses.

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Hydrocortisone, CAS 50-23-7

Pack Size	Availability	Price/USD	Quantity
500 mg	In stock	$ 45.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 99.95%

Purity: 99.83%

Purity: 99.71%

Purity: 98.98%

Purity: 98.4%

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Storage & Solubility Information

Description	Hydrocortisone (Cortisol) is a glucocorticoid hormone secreted by the adrenocortex. Hydrocortisone agonizes the glucocorticoid receptor, which promotes proteolytic metabolism, gluconeogenesis, capillary wall stabilization, and renal calcium excretion, as well as suppressing immune and inflammatory responses.
In vitro	METHODS: Human articular chondrocytes were cultured in medium containing Hydrocortisone (0.05-1.0 μg/mL), and the relevant indexes were measured by Flow Cytometry. RESULTS: The catabolic signaling pathway of chondrocytes treated with 0.05 μg/mL Hydrocortisone was reduced, and the ability to synthesize ECM macromolecules was enhanced. [1] METHODS: Porcine brain capillary endothelial cells PBCEC were cultured with medium containing Hydrocortisone (550 nM) for seven days, and the cytoskeleton was examined using fluorescence microscopy. RESULTS: Hydrocortisone-induced cytoskeletal rearrangement. [2]
In vivo	In ex vivo guinea pig hearts, Hydrocortisone reduces post-ischemic oxidative stress, perfusion pressure, and effusion formation. Within the human cerebral microvascular endothelial cell line hCMEC/D3, Hydrocortisone prevents endothelial barrier disruption induced by pro-inflammatory stimulation (administration of TNFα), partly by maintaining occludin levels. Hydrocortisone induces class-switch recombination from Sμ to Sε in IL-4-treated B cells, supporting a model for sequential isotype transformation from IgM to IgE through IgG4. Following dendritic cell treatment, Hydrocortisone decreases the expression of MHC class II molecules, the dendritic-specific marker CD83, and costimulatory molecule CD86, while significantly reducing IL-12 secretion. Additionally, it inhibits the increase of IL-4 (induced by IFN-γ) but does not affect IL-5. Hydrocortisone lowers T cell proliferation in dendritic cells. It prevents TNF-α-induced severe degradation of glycocalyx, increases in coronary resistance, vascular leakage and permeability, hydroxyethyl starch, and causes degranulation of mast cells in ex vivo guinea pig hearts. Hydrocortisone inhibits the shedding of multiligand glycosaminoglycans-1, heparan sulfate, and hyaluronic acid post-ischemia and suppresses the release of histamine from resident mast cells.
Cell Research	Cells are plated on top of collagen IV-coated transwell chambers for six-well plates (24 mm diameter, membrane material: polyethylene terephthalate (PET), 0.4 μm pores, pore density 1.6×106?cm2) at densities of 2.5×104?cells cm2?per well. When they have reached confluence at day 5, the different experimental sets of cells are transferred to differentiation medium containing reduced amounts of FCS and treated with TNFα or hydrocortisone as indicated.

Synonyms	Cortisol
Molecular Weight	362.46
Formula	C21H30O5
CAS No.	50-23-7

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 68 mg/mL (187.6 mM)

Ethanol: 22 mg/mL (60.7 mM)

References and Literature

1. Wang J, et al. Physiological levels of hydrocortisone maintain an optimal chondrocyte extracellular matrix metabolism. Ann Rheum Dis. 2004 Jan;63(1):61-6. 2. Schrot S, et al. Influence of hydrocortisone on the mechanical properties of the cerebral endothelium in vitro. Biophys J. 2005 Dec;89(6):3904-10. 3. Bergt S, et al. Hydrocortisone reduces the beneficial effects of toll-like receptor 2 deficiency on survival in a mouse model of polymicrobial sepsis. Shock. 2013 Nov;40(5):414-9. 4. Li Y, et al. Risk of death does not alter the efficacy of hydrocortisone therapy in a mouse E. coli pneumonia model: risk and corticosteroids in sepsis. Intensive Care Med. 2008 Mar;34(3):568-77. 5. Jabara HH, et al. J Immunol, 1993, 151(9), 4528-4533.

Citations

1. Lei H, Wang Z, Jiang D, et al. CRISPR screening identifies CDK12 as a conservative vulnerability of prostate cancer. Cell Death & Disease. 2021, 12(8): 1-11. 2. Li J, Bai Y, Liu Y, et al.Transcriptome-based chemical screens identify CDK8 as a common barrier in multiple cell reprogramming systems.Cell Reports.2023, 42(6). 3. Ji X, Jiang P, Li Y, et al.Maternal Bisphenol B Exposure and Mammary Gland Development of Offspring: A Time-Series Analysis.Environment & Health.2023

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Clinical Compound Library Anti-Cancer Approved Drug Library Anti-Cancer Drug Library EMA Approved Drug Library Drug Repurposing Compound Library Bioactive Compounds Library Max Approved Drug Library Clinical Compound Library FDA-Approved Drug Library Anti-Aging Compound Library

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Keywords

Hydrocortisone 50-23-7 Endocrinology/Hormones Metabolism Glucocorticoid Receptor Endogenous Metabolite Inhibitor inhibit Cortisol inhibitor

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