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KKL-35

Catalog No. T5331 CAS 865285-29-6

KKL-35 is an inhibitor of trans-translation (IC50: 0.9 μM) with broad-spectrum antibiotic activity.

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KKL-35, CAS 865285-29-6

Pack Size	Availability	Price/USD
1 mg	In stock	$ 72.00
2 mg	In stock	$ 98.00
5 mg	In stock	$ 179.00
10 mg	In stock	$ 273.00
25 mg	In stock	$ 463.00
50 mg	In stock	$ 668.00
100 mg	In stock	$ 933.00
1 mL * 10 mM (in DMSO)	In stock	$ 189.00

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Purity: 99.66%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	KKL-35 is an inhibitor of trans-translation (IC50: 0.9 μM) with broad-spectrum antibiotic activity.
Targets&IC50	Trans-translation tagging reaction:0.9 µM
In vitro	KKL-35 inhibited tagging of DHFR-ns. Dose-response experiments showed that KKL-35 inhibited tagging with an IC50 = 0.9 μM. KKL-35 prevented the growth of WT S. flexneri with a minimum inhibitory concentration (MIC) of 6 μM, and the addition of KKL-35 to a growing culture of S. flexneri stops growth [1]. Consistent with the essential nature of trans-translation in L. pneumophila, KKL-35 inhibited the growth of all tested strains at submicromolar concentrations. KKL-35 remained equally active against L. pneumophila mutants that have evolved resistance to macrolides. KKL-35 inhibited the multiplication of L. pneumophila in human macrophages at several stages of infection [2].
Cell Research	Overnight cultures of S. flexneri 2a 2457T, B. anthracis, M. smegmatis, JW5503, and NSR253 were grown in lysogeny broth (LB). Cultures were diluted to a final inoculum of ～5 × 10^5 cfu/mL in 96-well microtiter plates, compounds were added at appropriate concentrations, and growth was observed after 24-h incubation at 37 °C. The MIC was determined by the concentration of the compound in the last well that showed no bacterial growth. For MBC assays, 10 μL from wells containing the MIC, 2× MIC, and 4× MIC of each compound was diluted 10-fold, spotted on LB plates, and grown overnight at 37 °C. An inhibitor was scored as bactericidal if it resulted in 1,000-fold reduction in colony-forming units per milliliter from the original inoculum [1].

Molecular Weight	317.7
Formula	C15H9ClFN3O2
CAS No.	865285-29-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 8.33 mg/mL (26.23 mM), Sonication is recommended.

References and Literature

1. 1. Ramadoss NS, et al. Small molecule inhibitors of trans-translation have broad-spectrum antibiotic activity. Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10282-7. 2. Brunel R, et al. KKL-35 Exhibits Potent Antibiotic Activity against Legionella Species Independently of trans-Translation Inhibition. Antimicrob Agents Chemother. 2018 Jan 25;62(2). pii: e01459-17.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Bioactive Compounds Library Max Anti-Infection Compound Library NO PAINS Compound Library Drug-induced Liver Injury (DILI) Compound Library Anti-Bacterial Compound Library Bioactive Compound Library Immunology/Inflammation Compound Library Antibiotics Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

KKL-35 865285-29-6 Microbiology/Virology Antibacterial Bacterial Inhibitor KKL 35 inhibit KKL35 inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.