Home Tools

Cart

PIK-93

Catalog No. T2616 CAS 593960-11-3

Synonyms: PIK 93

PIK-93 is the first potent, synthetic PI4K inhibitor with IC50 of 19 nM; inhibits PI3Kα with IC50 of 39 nM.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

PIK-93, CAS 593960-11-3

Pack Size	Availability	Price/USD
1 mg	In stock	$ 37.00
5 mg	In stock	$ 82.00
10 mg	In stock	$ 122.00
25 mg	In stock	$ 222.00
50 mg	In stock	$ 372.00
100 mg	In stock	$ 549.00
1 mL * 10 mM (in DMSO)	In stock	$ 90.00

Bulk Inquiry

Select Batch

Purity: 99.83%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	PIK-93 is the first potent, synthetic PI4K inhibitor with IC50 of 19 nM; inhibits PI3Kα with IC50 of 39 nM.
Targets&IC50	PI3Kα:39 nM, PI4K:19 nM
In vivo	At concentrations ranging from 0.5 μM to 1 μM, PIK-93 impairs the stability of aggregates formed in differentiated HL60 cells treated with f-Met-Leu-Phe. In COS-7 cells, 250 nM of PIK-93 effectively eliminates the accumulation of the CERT-PH domain and FL-Cer in the Golgi apparatus. Additionally, in T6.11 cells, 300 nM of PIK-93 reduces Carbachol-induced translocation of TRPC6 to the plasma membrane and subsequent Ca2+ entry. The same concentration of PIK-93 also significantly inhibits the conversion of [3H]-labeled serine to endogenous sphingomyelin, indicating the critical role of PI4KIIIβ in the endoplasmic reticulum and Golgi apparatus for ceramide transfer and sphingomyelin synthesis regulation. Research demonstrates that PIK-93 possesses antiviral capabilities, inhibiting replication of the poliovirus and hepatitis C virus with EC50 values of 0.14 μM and 1.9 μM, respectively. PIK-93 effectively inhibits PI3Kγ and PI4KIIIβ, with IC50 values of 16 nM and 19 nM, respectively, and other PI3K members, including PI3Kα, β, and δ, with IC50 values of 39 nM, 0.59 μM, and 0.12 μM, while showing negligible inhibition effects on other kinases at concentrations up to 10 μM.
Kinase Assay	Assay of PI3Ks: IC50 values are measured using a standard TLC assay for lipid kinase activity. Kinase reactions are performed by preparing areaction mixture containing kinase, PIK-93 (2% DMSO final concentration), buffer (25 mM HEPES, pH 7.4, 10 mM MgCl2), and freshly sonicated phosphatidylinositol (100 μg/ml). Reactions are initiated by the addition of ATP containing 10 μCi of γ-32P-ATP to a final concentration 10 or 100 μM, and allowed to proceed for 20 min at room temperature. For TLC analysis, reactions are then terminated by the addition of 105 μL 1N HCl followed by 160 μL CHCl3:MeOH (1:1). The biphasic mixture is vortexed, briefly centrifuged, and the organic phase transferred to a new tube using a gel loading pipette tip precoated with CHCl3. This extract is spotted on TLC plates and developed for 3 hours–4 hours in a 65:35 solution of n-propanol:1M acetic acid. The TLC plates are then dried, exposed to a phosphorimager screen, and quantitated. Kinase activity is typically measured at 10–12 concentrations of PIK-93 representing two-fold dilutions from the highest concentration of 100 μM.
Cell Research	For actin staining, dHL60 cells are preincubated in suspension with PIK-93 or vehicle for 40 min, centrifuged for 5 min at 2000 rpm at room temperature in a J6-B centrifuge, resuspended in mHBSS containing the respective agent at the same concentration, allowed to stick to fibronectin-covered coverslips, and subjected to stimulation with a uniform concentration of 100 nM f-Met-Leu-Phe (fMLP) for 3 min. Cellsare fixed in 3.7% PFA and stained with 10 units/mL rhodamine-phalloidin for 15 min.(Only for Reference)

Synonyms	PIK 93
Molecular Weight	389.88
Formula	C14H16ClN3O4S2
CAS No.	593960-11-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 39 mg/mL (100 mM)

References and Literature

1. Knight ZA, Cell, 2006, 125(4), 733-747. 2. VanKeymeulen A, et al. Cell Biol. 2006, 174(3), 437-445. 3. Tóth B, et al. J BiolChem, 2006, 281(47), 36369-3637 4. Monet M, et al. J BiolChem, 2012, Epub ahead of print. 5. Arita M, et al. J Virol, 2011, 85(5), 2364-2372.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Kinase Inhibitor Library Anti-COVID-19 Compound Library Autophagy Compound Library Neural Regeneration Compound Library Cancer Cell Differentiation Compound Library Anti-Obesity Compound Library Bioactive Compound Library Anti-Diabetic Compound Library Anti-Cancer Compound Library

Related Products

Related compounds with same targets

A011 Torin 2 ETP-46464 AZD0156 Elimusertib hydrochloride(1876467-74-1 free base) ATR-IN-29 ATM Inhibitor-1 KU-60019

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

PIK-93 593960-11-3 DNA Damage/DNA Repair Metabolism Microbiology/Virology PI3K/Akt/mTOR signaling Virus Protease ATM/ATR DNA-PK Carbonic Anhydrase mTOR PI4K HSV PI3K Phosphatidylinositol 4 kinases PI4 kinases Phosphoinositide 3-kinase inhibit Inhibitor PIK93 PIK 93 inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.