Home Tools

Pirfenidone

Catalog No. T2386 CAS 53179-13-8

Synonyms: S-7701,AMR-69, S-7701, AMR69

Pirfenidone (AMR69) is an inhibitor for TGF-β production and TGF-β stimulated collagen production. Pirfenidone inhibits fibroblast, epidermal, platelet-derived, and transforming beta-1 growth factors, thereby slowing tumor cell proliferation. This agent also inhibits DNA synthesis and the production of mRNA for collagen types I and III, resulting in a reduction in radiation-induced fibrosis.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Pirfenidone, CAS 53179-13-8

Pack Size	Availability	Price/USD
25 mg	In stock	$ 30.00
50 mg	In stock	$ 39.00
100 mg	In stock	$ 57.00
200 mg	In stock	$ 73.00
500 mg	In stock	$ 97.00
1 g	In stock	$ 147.00
5 g	In stock	$ 217.00
1 mL * 10 mM (in DMSO)	In stock	$ 39.00

Bulk Inquiry

Select Batch

Purity: 99.93%

Purity: 99.9%

Purity: 99.88%

Purity: 99.61%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Pirfenidone (AMR69) is an inhibitor for TGF-β production and TGF-β stimulated collagen production. Pirfenidone inhibits fibroblast, epidermal, platelet-derived, and transforming beta-1 growth factors, thereby slowing tumor cell proliferation. This agent also inhibits DNA synthesis and the production of mRNA for collagen types I and III, resulting in a reduction in radiation-induced fibrosis.
In vitro	Treatment with 0.5% Pirfenidone has been shown to reduce rat liver injury, a process linked to diminished proliferation of hepatic stellate cells and reduced collagen deposition. This treatment also leads to a 50-60% decrease in the transcription levels of procollagen α1(I), TIMP-1, and MMP-2 in rats induced with dimethylnitrosamine. In mice, Pirfenidone (250 mg/kg) significantly inhibits the production of pro-inflammatory cytokines including TNF-α, INF-γ, and IL-6, while promoting the production of the anti-inflammatory cytokine IL-10. In Sprague-Dawley rats on a low-sodium diet, Pirfenidone (250 mg/kg/day) ameliorates about 50% of cyclosporine-induced fibrosis and reduces the expression of TGF-β1 protein by 80%. Additionally, in ICR mice with bleomycin-induced pulmonary fibrosis receiving daily intravenous injections, Pirfenidone (400 mg/kg) suppresses HSP47-positive cells and myofibroblasts, key contributors to extracellular matrix accumulation.
In vivo	In CCL-64 cells, Pirfenidone (< 5 mM) reduces TGF-β bioactivity by affecting the expression of TGF-β2 mRNA and the pro-TGF-β process. In LN-308 cells, Pirfenidone (< 8.3 mM) exhibits dose-dependent inhibition of recombinant plasminogen activator activity and MMP-11 expression. Across LN-18, T98g, LNT-229, and LN-308 cell lines, Pirfenidone (<10 mM) concentration-dependently decreases glioma cell density. In RAW264.7 cells, Pirfenidone (< 300 μg/mL) inhibits TNF-α through a translational mechanism, a process unrelated to the activation of mitogen-activated protein kinase 2, p38MAP kinase, and JNK.
Cell Research	Pirfenidone (PFD) is dissolved in DMSO and stored, and then diluted with appropriate media before use[3]. HLECs are seeded in 96-well plates (1×104 cells/well) for 24 hours in α-MEM/10% FBS/1%NEAA, and are cultured in stationary tubes in serum-free medium for 24 hours. And then the culture medium is removed and cells are bathed in α-MEM with 10% FBS and 1% NEAA supplemented with 0, 0.01, 0.1, 0.2, 0.3, 0.5, or 1 mg/mL Pirfenidone for 0, 4, 12, 24, 48, or 72 hours. After incubation with 180 μL α-MEM and 20 μL of 5 mg/mL MTT for 4 hours at 37°C, the MTT solution is discarded. The Formosan precipitates are dissolved in 180 μL DMSO by agitating the dishes for 10 minutes at 200 rpm on an orbital shaker. The absorbance at 490 nm in each well is read with a micro plate reader. We further examined the effects of PFD by refining the concentrations at 0.2, 0.25, 0.3, 0.4, 0.5 and 0.6 mg/mL using the MTT assay[3].

Synonyms	S-7701,AMR-69, S-7701, AMR69
Molecular Weight	185.22
Formula	C12H11NO
CAS No.	53179-13-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 100mg/ml (539.9mM)

H2O: 25 mg/ml （134.96mM）

References and Literature

1. Nakazato H, et al. Eur J Pharmacol, 2002, 446(1-3), 177-185. 2. Burghardt I, et al. Biochem Biophys Res Commun, 2007, 354(2), 542-547. 3. Shihab FS, et al. Am J Transplant, 2002, 2(2), 111-119. 4. Kakugawa T, et al. Eur Respir J, 2004, 24(1), 57-65. 5. Di Sario A, et al. Dig Liver Dis, 2004, 36(11), 744-751.

Citations

1. Hong M J, Hao M J, Zhang G Y, et al. Exophilone, a Tetrahydrocarbazol-1-one Analogue with Anti-Pulmonary Fibrosis Activity from the Deep-Sea Fungus Exophiala oligosperma MCCC 3A01264. Marine Drugs. 2022, 20(7): 448 2. Liang H X, Hao M J, Zhang G Y, et al.Lentinuses A–B, two alkaloids from the marine-derived fungus Lentinus sajor-caju with potent anti-pulmonary fibrosis activity.Fitoterapia.2023: 105433. 3. Tian J, Song D, Peng Y, et al.Silica-induced macrophage pyroptosis propels pulmonary fibrosis through coordinated activation of relaxin and osteoclast differentiation signaling to reprogram fibroblasts.Ecotoxicology and Environmental Safety.2024, 273: 116106.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Clinical Compound Library Anti-Cancer Approved Drug Library Membrane Protein-targeted Compound Library Anti-Cancer Drug Library Inhibitor Library TGF-beta/Smad Compound Library Drug Repurposing Compound Library Anti-Cancer Active Compound Library EMA Approved Drug Library Drug-Fragment Library

Related Products

Related compounds with same targets

RS102895 hydrochloride Maraviroc AZD2098 AZD-5672 CCX140 SB297006 CCR2-RA-[R] PF-4136309

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Pirfenidone 53179-13-8 Immunology/Inflammation Microbiology/Virology Stem Cells CCR TGF-beta/Smad S-7701,AMR-69 AMR 69 CC chemokine receptor Transforming growth factor beta S 7701 S-7701 AMR-69 S7701 Inhibitor AMR69 inhibit inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.