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Tunicamycin

Catalog No. T13229 CAS 11089-65-9

Tunicamycin is a mixture of antibiotics that inhibit N-linked glycosylation by blocking GlcNAc phosphotransferase (GPT). Tunicamycin has antitumor activity, as well as anti-bacterial, anti-fungal, and anti-viral activity.

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Tunicamycin, CAS 11089-65-9

Pack Size	Availability	Price/USD
1 mg	In stock	$ 105.00
2 mg	In stock	$ 155.00
5 mg	In stock	$ 318.00
10 mg	In stock	$ 538.00
25 mg	In stock	$ 857.00
50 mg	In stock	Inquiry

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Purity: 98.77%

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Description	Tunicamycin is a mixture of antibiotics that inhibit N-linked glycosylation by blocking GlcNAc phosphotransferase (GPT). Tunicamycin has antitumor activity, as well as anti-bacterial, anti-fungal, and anti-viral activity.
In vitro	METHODS: Human hepatocellular carcinoma cells Hep3B were treated with Tunicamycin (1 μg/mL), camptothecin (3 μM), etoposide (5 μM), taxol (0.1 μM), and vincristine (0.1 μM) for 48 h, and cell death was detected by Flow Cytometry. RESULTS: Tunicamycin significantly inhibited apoptosis induced by TOP inhibitors (camptothecin and etoposide) but not by microtubule-targeting drugs (taxol and vincristine). [1] METHODS: Human hepatocellular carcinoma cells PLC/PRF/5, MHCC-97L and MHCC-97H were treated with Tunicamycin (2.5 μg/mL) for 24 h, and the expression levels of the target proteins were detected by Western Blot. RESULTS: Tunicamycin inhibited the phosphorylation of Akt in the three hepatocellular carcinoma cell lines. [2]
In vivo	METHODS: To investigate the effects on hepatic energy metabolism, Tunicamycin (1 mg/kg) was administered intraperitoneally to C57BL/6 mice as a single injection. RESULTS: Tunicamycin significantly induced hepatic yellow coloration and endoplasmic reticulum stress, and increased serum aspartate aminotransferase and alanine aminotransferase levels.Tunicamycin altered hepatic energy homeostasis by increasing triglyceride accumulation and decreasing glycogen content. [3] METHODS: To test the antitumor activity in vivo, Tunicamycin (0.25 mg/kg) was administered orally twice a week for four weeks to Balb/c (nu/nu) mice harboring human triple-negative breast carcinoma tumor MDA-MB-231. RESULTS: Within one week of oral administration of Tunicamycin, MDA-MB-231 tumor xenografts were reduced by 65% and there was no systemic and/or organ failure. [4]

Molecular Weight	844.94 (n=10)
Formula	C39H64N4O16
CAS No.	11089-65-9

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 14.37 mg/mL (20 mM), Sonication is recommended.

References and Literature

1. Hsu JL, et al. Tunicamycin induces resistance to camptothecin and etoposide in human hepatocellular carcinoma cells: role of cell-cycle arrest and GRP78. Naunyn Schmiedebergs Arch Pharmacol. 2009 Nov;380(5):373-82. 2. Hou H, et al. Tunicamycin potentiates cisplatin anticancer efficacy through the DPAGT1/Akt/ABCG2 pathway in mouse Xenograft models of human hepatocellular carcinoma. Mol Cancer Ther. 2013 Dec;12(12):2874-84. 3. Feng B, et al. Endoplasmic Reticulum Stress Inducer Tunicamycin Alters Hepatic Energy Homeostasis in Mice. Int J Mol Sci. 2017 Aug 4;18(8):1710. 4. Banerjee A, et al. Unfolded protein response is required in nu/nu mice microvasculature for treating breast tumor with tunicamycin. J Biol Chem. 2011 Aug 19;286(33):29127-29138.

Citations

1. Dai W, Wang K, Zhen X, et al. Magnesium isoglycyrrhizinate attenuates acute alcohol-induced hepatic steatosis in a zebrafish model by regulating lipid metabolism and ER stress. Nutrition & Metabolism. 2022, 19(1): 1-12.

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Keywords

Tunicamycin 11089-65-9 Microbiology/Virology Influenza Virus Antibacterial Antibiotic Antifungal N-linked DNA UDP-HexNAc inhibit anti-cancer Inhibitor ATF6 synthesis invasion XBP-1 Bacterial Fungal glycoprotein inhibitor

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