Linoleic acid (9,12-octadecadienoic acid) is a naturally occurring polyunsaturated fatty acid found in animal and vegetable oils. Linoleic acid is an essential fatty acid that is part of membrane phospholipids.

METHODS: Bovine satellite cell BSCs were treated with Linoleic acid (10-250 µM) for 24-48 h. Cell viability was measured by MTT assay.
RESULTS: At 24 h of treatment with Linoleic acid, cell proliferation increased at doses of 10-100 µM and decreased at 250 µM as compared to untreated control. 100 µM Linoleic acid at 48 h had no significant effect on cell proliferation. [1]
METHODS: Human ovarian cancer cells SKOV-3 were treated with Linoleic acid (16 µM) for 24 h. Cell migration was detected by wound-healing assay.
RESULTS: The average migration in the Linoleic acid treated group increased by about 20% on average compared to control untreated cells. [2]

METHODS: To study the effects on endocannabinoid (EC) biology, four groups of C57BL/6 mice were fed diets containing 1% or 8% Linoleic acid (with or without G (LAG)) for eight weeks.
RESULTS: Increasing dietary Linoleic acid from 1% to 8% significantly increased circulatory, small intestinal, and hepatic ECS. 1% LAG-fed mice had the lowest feed efficiencies, and hepatic levels of only two ECs were reduced by the addition of G. [3]