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Methotrexate metabolite

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Purity:99.7%

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COA HNMR HPLC

Methotrexate metabolite

Catalog No. T16045Cas No. 19741-14-1

Methotrexate metabolite (DAMPA) (DAMPA) is the active metabolite of Methotrexate. Methotrexate is a folic acid antagonist. Methotrexate is used as a chemotherapeutic agent and immunosuppressant.

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Pack Size	Price	Availability
10 mg	$39	In Stock
25 mg	$80	In Stock
50 mg	$128	In Stock
100 mg	$193	In Stock
200 mg	$287	In Stock
500 mg	$486	In Stock
1 mL x 10 mM (in DMSO)	$39	In Stock

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Product Introduction

Bioactivity

Description	Methotrexate metabolite (DAMPA) (DAMPA) is the active metabolite of Methotrexate. Methotrexate is a folic acid antagonist. Methotrexate is used as a chemotherapeutic agent and immunosuppressant.
In vitro	Methotrexate metabolite was the most active, with 50% inhibitory concentrations in vitro of 446 ng/ml against the antifolate-sensitive strain and 812 ng/ml against the highly resistant strain under physiological folate conditions. Methotrexate metabolite potentiates the activity of the sulfone dapsone, an inhibitor of dihydropteroate synthase, but not that of chlorcycloguanil, a known inhibitor of dihydrofolate reductase (DHFR)[2].
Alias	DAMPA

Chemical Properties

Molecular Weight	325.33
Formula	C15H15N7O2
Cas No.	19741-14-1

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 4 mg/mL (12.30 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.0738 mL	15.3690 mL	30.7380 mL	153.6901 mL
5 mM	0.6148 mL	3.0738 mL	6.1476 mL	30.7380 mL
10 mM	0.3074 mL	1.5369 mL	3.0738 mL	15.3690 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

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A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

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Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

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