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MMAF

Name: MMAF
Brand: TargetMol
SKU: T3256
Price: 40 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3256Cas No. 745017-94-1

Alias MonoMethyl auristatin F

MMAF (MonoMethyl auristatin F), an antimitotic agent, inhibits cell division by blocking the polymerization of tubulin.

MMAF

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Purity: 98.48%

Catalog No. T3256Alias MonoMethyl auristatin FCas No. 745017-94-1

MMAF (MonoMethyl auristatin F), an antimitotic agent, inhibits cell division by blocking the polymerization of tubulin.

Pack Size	Price	Availability
5 mg	$40	In Stock
10 mg	$56	In Stock
25 mg	$90	In Stock
50 mg	$129	In Stock
100 mg	$197	In Stock
200 mg	$293	In Stock

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Purity:98.48%

Resource Download

COA HNMR HPLC

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Product Introduction

Bioactivity

Description	MMAF (MonoMethyl auristatin F), an antimitotic agent, inhibits cell division by blocking the polymerization of tubulin.
Targets&IC50	H3397:105 nM.
In vitro	MMAF shows in vitro cytotoxicity against a panel of cell lines. The IC50 values for Karpas 299, H3396, 786-O and Caki-1 are 119, 105, 257, and 200 nM, respectively. Targeted MMAF is much more potent than the free drug, and that cAC10 conjugates of MMAF display pronounced activities. On a molar basis, the cAC10-L1-MMAF4 is an average of over 2200-fold more potent than free MMAF and is active on all the CD30-positive cell lines tested[1].
In vivo	The maximum tolerated dose in mice of MMAF (>16 mg/kg) is much higher than MMAE (1 mg/kg). cAC10-L1-MMAF4 has an MTD of 50 mg/kg in mice and 15 mg/kg in rats. The corresponding cAC10-L4-MMAF4 ADC was much less toxic, having MTDs in mice and rats of >150 mg/ kg and 90 mg/kg in rats, respectively[1].
Cell Research	Cells are treated with serial dilutions of test molecules and incubated 4-6 days depending on cell line. Assessment of cellular growth and data reduction to generate IC50 values is done using Alamar Blue dye reduction assay[2].
Alias	MonoMethyl auristatin F

Chemical Properties

Molecular Weight	731.96
Formula	C₃₉H₆₅N₅O₈
Cas No.	745017-94-1
Smiles	CC[C@H](C)C([C@@H](CC(=O)N1CCC[C@H]1[C@H](OC)[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(O)=O)OC)N(C)C(=O)[C@@H](NC(=O)[C@@H](NC)C(C)C)C(C)C
Relative Density.	1.116 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 20 mg/mL (27.32 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.3662 mL	6.8310 mL	13.6619 mL	68.3097 mL
5 mM	0.2732 mL	1.3662 mL	2.7324 mL	13.6619 mL
10 mM	0.1366 mL	0.6831 mL	1.3662 mL	6.8310 mL
20 mM	0.0683 mL	0.3415 mL	0.6831 mL	3.4155 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc