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BMS-265246

Catalog No. T2679 CAS 582315-72-8

Synonyms: BMS265246

BMS-265246 is a potent and selective CDK1/2 inhibitor.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

BMS-265246, CAS 582315-72-8

Pack Size	Availability	Price/USD
1 mg	In stock	$ 47.00
5 mg	In stock	$ 113.00
10 mg	In stock	$ 197.00
25 mg	In stock	$ 389.00
50 mg	In stock	$ 589.00
100 mg	In stock	$ 843.00
500 mg	In stock	$ 1,730.00
1 mL * 10 mM (in DMSO)	In stock	$ 125.00

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Purity: 99.55%

Purity: 98.77%

Purity: 98.33%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	BMS-265246 is a potent and selective CDK1/2 inhibitor.
Targets&IC50	CDK4-CyclinD:230 nM, CDK2-CyclinE:9 nM, CDK1-CyclinB:6 nM
Kinase Assay	CDK1/Cyclin B1 Kinase Assay: Kinase reactions consists of 100 ng of baculovirus expressed GST-CDK1/cyclin B1 complex, 1 μg histone H1, 0.2 μCi 33P γ-ATP, 25 μM ATP in 50 μL of kinase buffer (50 mM Tris, pH 8.0, 10 mM MgCl2, 1 mM EGTA, 0.5 mM DTT). Reactions are incubated for 45 min at 30 °C and stopped by the addition of cold trichloroacetic acid (TCA) to a final concentration of 15%. TCA precipitates are collected onto GF/C unifilter plates using a Filtermate universal harvester, and the filters are quantitated using a TopCount 96 well liquid scintillation counter. Dose response curves are generated to determine the concentration required to inhibit 50% of kinase activity (IC50). BMS265246 is dissolved at 10 mM in DMSO and evaluated at six concentrations, each in triplicate. The final concentration of DMSO in the assay equals 2%. IC50 values are derived by nonlinear regression analysis and have a coefficient of variance (SD/mean, n = 6) = 16%.
Cell Research	HCT-116 cells are plated onto 96-well dishes. For each well, the cell density is calculated by counting the number of objects (cells) per field of view, and averaging across all fields for a given well. For a treatment compound, cell density is converted to a percentage relative to the plate-averaged cell density from DMSO treatment (i.e., 100% corresponds to the average cell density for DMSO treatment). Logistic regression curve fits are done using TIBCO Spotfire, and the concentration at which the curve crosses 50% is reported as the EC50 of BMS-265246.(Only for Reference)

Synonyms	BMS265246
Molecular Weight	345.34
Formula	C18H17F2N3O2
CAS No.	582315-72-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 16 mg/mL (46.3 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Misra RN, et al. Bioorg Med Chem Lett. 2003, 13(14), 2405-2408. 2. Sutherland JJ, et al. Mol Cancer Ther. 2011, 10(2), 242-254.

Citations

1. Jiang L, Yu Y, Li Z, et al.BMS-265246, a Cyclin-Dependent Kinase Inhibitor, Inhibits the Infection of Herpes Simplex Virus Type 1.Viruses.2023, 15(8): 1642.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Cancer Active Compound Library Anti-Breast Cancer Compound Library Kinase Inhibitor Library Anti-Cancer Compound Library NO PAINS Compound Library Cell Cycle Compound Library Anti-Aging Compound Library Anti-Prostate Cancer Compound Library Anti-Pancreatic Cancer Compound Library

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Mevociclib FMF-04-159-2 BI-1347 TBB AZA1 Eciruciclib WHI-P180 Leucettinib-92

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

BMS-265246 582315-72-8 Cell Cycle/Checkpoint CDK Pyrazolopyridine Inhibitor iTreg cell A2780 Angiotensin-converting Enzyme (ACE) MCF7 cells Cyclin dependent kinase inhibit CHI3L1 ovarian cancer BMS265246 Calu-3 cells BMS 265246 COVID-19 inhibitor

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