BODIPY 581/591 C11 is a fluorescent radioactive probe used to indicate lipid peroxidation and antioxidant efficacy in model membrane systems and living cells.

I. Preparation of stock solution
Dissolve 1 mg BODIPY 581/591 C11 in 0.1983 mL DMSO to obtain 10 mM BODIPY 581/591 C11.
Note: Store the stock solution at -20°C or -80°C away from light and avoid repeated freezing and thawing.
Preparation of BODIPY 581/591 C11 working solution
Dilute the stock solution with pure DMEM medium or PBS to make BODIPY 581/591 C11 working solution at 1-10 μM.
Note: Please adjust the concentration of BODIPY 581/591 C11 Working Solution according to your experimental plan and practice.
Cell Staining
1) Preparation requirements for different cell types:
Suspension cells: centrifuge at 4℃, 1000g for 3-5 minutes, discard the supernatant. wash with PBS twice for 5 minutes each time.
Adherent cells: discard the cell culture medium, add trypsin (1 ½ min) to dissociate the cells and make a single cell suspension. Centrifuge at 1000 g for 3-5 minutes at 4°C, discard supernatant and wash twice with PBS for 5 minutes each time.
Note: Please be gentle when using PBS cells and when blowing the cells with the pipette gun.
2) Add 1 mL of BODIPY 581/591 C11 working solution to the prepared cells and incubate for 30 minutes at room temperature.
3) Centrifuge at 400 g for 4 minutes at 4°C and discard the supernatant.
4) Wash with PBS 2 times for 5 minutes each time.
5) Resuspend the cells using pure DMEM medium or PBS, and observe the stained cells by fluorescence microscope or flow cytometer.
Notes:
1. This product is intended for research and development use only and should not be used for medication, household or other purposes.
2. For your safety and health, please wear a white coat and disposable gloves to operate.