Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Betulinic acid (ALS-357) is a pentacyclic lupane-type triterpene derivative of betulin (isolated from the bark of Betula alba, the common white birch) which has antiretroviral, antimalarial, and anti-inflammatory properties, as well as a more recently discovered potential as an anticancer agent, by inhibition of topoisomerase.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 mg | In stock | $ 33.00 | |
100 mg | In stock | $ 48.00 | |
500 mg | In stock | $ 174.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 54.00 |
Description | Betulinic acid (ALS-357) is a pentacyclic lupane-type triterpene derivative of betulin (isolated from the bark of Betula alba, the common white birch) which has antiretroviral, antimalarial, and anti-inflammatory properties, as well as a more recently discovered potential as an anticancer agent, by inhibition of topoisomerase. |
Targets&IC50 | HIV-1:1.4 μM(EC50), Aminopeptidase N:7.3 μM, Eukaryotic topoisomerase I:5 μM |
In vitro | In LNCaP cell cultures of athymic nude mice, Betulinic acid inhibits the growth of prostate cancer cells and tumors (xenografts). This inhibitory effect is partly due to the downregulation of protease-dependent Sp1/3/4 and several SP-regulated genes. |
In vivo | As an anti-melanoma agent, Betulinic acid inhibits the activity of aminopeptidase N (IC50: 7.3 μM). It exhibits anti-HIV properties by blocking HIV replication in H9 lymphocytes (EC50: 1.4 μM) and inhibiting the growth of uninfected H9 cells (IC50: 13 μM). Moreover, Betulinic acid shows potential as an anti-cancer candidate, suppressing the activity of eukaryotic topoisomerase I (IC50: 5 μM). |
Kinase Assay | Topoisomerase I-DNA interaction: Annealed 25-mer duplex of oligonucleotide-1 and oligonicleotide-2 is incubated with 25 or 50 units of rat liver topoisomerase I at 8 °C for 15 min in the presence or absence of betulinic acid in binding buffer. After incubation the reaction mixtures are electrophoresed in 7% non-denaturing polyacrylamide gel at 4 °C in 0.167 × TBE buffer and DNA bands are stained with ethidium bromid |
Source |
Synonyms | ALS-357, Lupatic acid, Betulic acid |
Molecular Weight | 456.7 |
Formula | C30H48O3 |
CAS No. | 472-15-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 10 mg/mL (21.89 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 16 mg/mL (35 mM)
You can also refer to dose conversion for different animals. More
bottom
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Betulinic acid 472-15-1 Apoptosis Autophagy DNA Damage/DNA Repair Metabolism Microbiology/Virology NF-Κb Proteases/Proteasome NF-κB Mitophagy Aminopeptidase Topoisomerase Endogenous Metabolite HIV Protease Human immunodeficiency virus HIV Nuclear factor-kappaB inhibit Nuclear factor-κB ALS357 Inhibitor Mitochondrial Autophagy ALS-357 Lupatic acid ALS 357 Betulic acid inhibitor