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Proscillaridin A

Catalog No. TN2105 CAS 466-06-8

Proscillaridin A is a natural product.It is a potent poison of topoisomerase I/II activity with IC50 values of 30 nM and 100 nM, respectively.

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Proscillaridin A, CAS 466-06-8

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Purity: 98.38%

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Description	Proscillaridin A is a natural product.It is a potent poison of topoisomerase I/II activity with IC50 values of 30 nM and 100 nM, respectively.
Targets&IC50	Topo I:30 nM , Topo II:100 nM
In vitro	proscillaridin A, on the proliferation of breast cancer MCF-7 cells.?In terms of inhibition of cell proliferation of MCF-7 cells, the compounds rank in the order proscillaridin A>digoxin>ouabain.?While both digoxin and ouabain inhibited topoisomerase II catalytic activity at nanomolar concentrations (100 nM), neither agent inhibited topoisomerase I catalytic activity even at concentrations as high as 100 microM.?On the other hand, proscillaridin A was a potent poison of topoisomerase I and II activity at nanomolar drug concentrations (30 nM, 100 nM, respectively), suggesting that this agent may produce its cytotoxic activity by targeting both enzymes simultaneously.?These studies suggest that the stabilization of DNA-topoisomerase II complexes is closely linked to the mechanism of digoxin, ouabain and proscillaridin A cytotoxicity.?The potential DNA-binding properties of the cardiac glycosides have been assessed by measuring the displacement of ethidium bromide from calf thymus DNA.
Source	Natural Products > Asparagaceae > Drimia

Molecular Weight	530.65
Formula	C30H42O8
CAS No.	466-06-8

Storage

store at low temperature

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 100 mg/mL (188.45 mM), Sonication is recommended.

References and Literature

1. Bielawski K, et al. Inhibition of DNA topoisomerases I and II, and growth inhibition of breast cancer MCF-7 cells by ouabain, digoxin and proscillaridin A.Biol Pharm Bull. 2006 Jul;29(7):1493-7.

Related compound libraries

This product is contained In the following compound libraries：

Selected Plant-Sourced Compound Library Rare Natural Compound Library

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Keywords

Proscillaridin A 466-06-8 DNA Damage/DNA Repair Topoisomerase inhibit MCF-7 breast cancer cell Inhibitor inhibitor

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