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SCR7 pyrazine

Catalog No. T1724 CAS 14892-97-8

Synonyms: SCR7

SCR7 pyrazine (SCR7) enhances CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency in vitro up to 19-fold. Inhibits nonhomologous end-joining (NHEJ).

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SCR7 pyrazine, CAS 14892-97-8

Pack Size	Availability	Price/USD
1 mg	In stock	$ 33.00
2 mg	In stock	$ 46.00
5 mg	In stock	$ 72.00
10 mg	In stock	$ 123.00
25 mg	In stock	$ 271.00
50 mg	In stock	$ 429.00
100 mg	In stock	$ 619.00
1 mL * 10 mM (in DMSO)	In stock	$ 72.00

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Purity: 99.85%

Purity: 99.83%

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Description	SCR7 pyrazine (SCR7) enhances CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency in vitro up to 19-fold. Inhibits nonhomologous end-joining (NHEJ).
In vitro	SCR7 effectively inhibits the formation of multimers at 200 μM and above. SCR7 successfully inhibits cell proliferation of MCF7, A549, HeLa, T47D, A2780, HT1080, and Nalm6 with IC50 of 40, 34, 44, 8.5, 120, 10, and 50 μM, respectively.[1] SCR7 suppresses the NHEJ repair of CRISPR-Cas9-induced DSBs.[2]
In vivo	SCR7 treatment (10 mg/kg, i.m.) signi?cantly reduces breast adenocarcinoma-induced tumor, and exhibits 4-fold increase in lifespan compared with control group. However, in Swiss albino mice with Dalton's lymphoma tumor model, SCR7 (20 mg/kg, i.p.) exhibits neither tumor regression nor increase in lifespan. In BALB/c mice, SCR7 (20 mg/kg, i.p.) signi?cantly enhances the cytotoxic effects of radiation, etoposide and 3-Aminobenzamide on tumor derived from Dalton's lymphoma (DLA) cells.[1]
Kinase Assay	Complementation of SCR7 Inhibition with Puri?ed Ligase IV: Complementation experiment is carried out by adding increasing concentrations of puri?ed Ligase IV/XRCC4 complex (30, 60, and 120 fmol) along with the oligomeric DNA substrates (5' compatible and 5'-5' noncompatible ends) to the SCR7-treatedextracts. Reactions are incubated for 2 h at 25℃. The reaction products are then resolved on 8% denaturing PAGE. The gel is dried and exposed and the signal is detected with a PhosphorImager and analyzed with Multi Gauge (V3.0) software.
Cell Research	Cell proliferation of cancer cells are determined by MTT and trypan blue assays. Briefly, MCF7, CEM, HeLa, A549, HT1080, A2780, T47D, Nalm6, N114 and K562 cells are grown in presence of SCR7 (10, 50, 100, and 250?μM) for 24 or 48?h, and subjected to MTT or trypan blue assays. Each experiment is repeated a minimum of three independent times. (Only for Reference)

Synonyms	SCR7
Molecular Weight	332.38
Formula	C18H12N4OS
CAS No.	14892-97-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 3 mg/mL (9.03 mM)

DMSO: 66 mg/mL (198.57 mM)

References and Literature

1. Srivastava M, et al. Cell. 2012, 151(7), 1474-1487. 2. Chu VT, et al. Nat Biotechnol. 2015, doi: 10.1038/nbt.3198.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Cancer Active Compound Library Bioactive Compounds Library Max Anti-Aging Compound Library Target-Focused Phenotypic Screening Library Apoptosis Compound Library Cell Cycle Compound Library Toxic Compound Library NO PAINS Compound Library DNA Damage & Repair Compound Library

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Keywords

SCR7 pyrazine 14892-97-8 Apoptosis Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA/RNA Synthesis CRISPR/Cas9 SCR-7 pyrazine Caspase 9 ligase-IV nonhomologous Caspase 3 SCR-7 DNA Inhibitor SCR7 SCR 7 replacement anticancer gene end-joining phosphorylation inhibit inhibitor

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