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Spermidine

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Purity:99.21%

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COA GC HNMR

Spermidine

Catalog No. T4893Cas No. 124-20-9

Spermidine (N-(3-Aminopropyl)-1,4-diaminobutane) inhibits NOS1 (nNOS). Spermidine binds and precipitates DNA and may be used for purification of DNA binding proteins. Spermidine activates PNK (polynucleotide kinase T4). Spermidine binds to and activates NMDA and has been shown to potentiate NMDA-induced currents in a concentration-dependent manner.

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Pack Size	Price	Availability	Quantity
100 mg	$45	In Stock

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Product Introduction

Bioactivity

Description	Spermidine (N-(3-Aminopropyl)-1,4-diaminobutane) inhibits NOS1 (nNOS). Spermidine binds and precipitates DNA and may be used for purification of DNA binding proteins. Spermidine activates PNK (polynucleotide kinase T4). Spermidine binds to and activates NMDA and has been shown to potentiate NMDA-induced currents in a concentration-dependent manner.
In vitro	METHODS: Human neuroblastoma cells SH-SY5Y were treated with Spermidine (0.05-10 µM) for 24-48 h. Mitochondrial metabolic activity was measured by MTT assay and ATP production assay. RESULTS: No effect of Spermidine was observed after 24 h. 0.1 and 1 µM Spermidine significantly increased cellular metabolic activity and ATP production after 48 h of treatment. The most effective concentration of Spermidine, 0.1 µM, induced an 8.2% increase in metabolic activity and a 4% increase in ATP production. [1] METHODS: The retinal pigment epithelial cell line ARPE-19 was treated with H2O2 (300 µM) and Spermidine (10 µM) for 24 h. Apoptosis was detected by Flow cytometry. RESULTS: 300 µM H2O2 significantly increased the frequency of annexin V-positive cells to approximately 25%, but Spermidine significantly inhibited this increase. The results suggest that Spermidine attenuates H2O2-mediated oxidative stress-induced apoptosis in ARPE-19 cells. [2]
In vivo	METHODS: To study the effects on diabetes, Spermidine (10 mM) was administered orally in water to non-obese diabetic (NOD) mice (type 1 diabetes mouse model) for thirty weeks. RESULTS: Treatment of NOD mice with Spermidine resulted in a higher incidence of diabetes, although pancreatic insulitis was not altered.Spermidine modulated pancreatic tissue polyamine levels and increased signs of autophagy.Spermidine resulted in an increase in the proportion of pro-inflammatory T-cells in the pancreatic lymph nodes (pLN) of diabetic mice. [3]
Alias	N-(3-Aminopropyl)-1,4-diaminobutane, N-(4-Aminobutyl)-1,3-diaminopropane

Chemical Properties

Molecular Weight	145.25
Formula	C7H19N3
Cas No.	124-20-9

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

5% DMSO+95% Saline: 1.67 mg/mL (11.47 mM)

DMSO: 33.33 mg/mL (229.47 mM)

Solution Preparation Table

DMSO/5% DMSO+95% Saline

	1mg	5mg	10mg	50mg
1 mM	6.8847 mL	34.4234 mL	68.8468 mL	344.2341 mL
5 mM	1.3769 mL	6.8847 mL	13.7694 mL	68.8468 mL
10 mM	0.6885 mL	3.4423 mL	6.8847 mL	34.4234 mL

DMSO

	1mg	5mg	10mg	50mg
20 mM	0.3442 mL	1.7212 mL	3.4423 mL	17.2117 mL
50 mM	0.1377 mL	0.6885 mL	1.3769 mL	6.8847 mL
100 mM	0.0688 mL	0.3442 mL	0.6885 mL	3.4423 mL

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