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Streptozocin

Catalog No. T1507 CAS 18883-66-4

Synonyms: STZ, NSC-85998, U 9889, Streptozotocin

Streptozotocin is an Antibiotic that is produced by Stretomyces achromogenes. It is used as an antineoplastic agent and to induce diabetes in experimental animals.

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Streptozocin, CAS 18883-66-4

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Streptozotocin is an antibiotic that is produced by Stretomyces achromogenes. It is used as an antineoplastic agent and to induce diabetes in experimental animals.
Targets&IC50	DNA-methylating (K562 cell):904 μg/mL, DNA-methylating (HL60 cell):11.7 μg/mL, DNA-methylating (C1498 cell):1024 μg/mL
Cell Research	Streptozocin (STZ) stock solutions of 50 mg/mL in distilled Water (dWater) are freshly prepared for each experiment[2]. Human and murine cell lines are cultured in triplicate in 96-well plates at a density of 2×104 cells/well in the absence (untreated control) or presence of various concentrations of ALX (20-3000 μg/mL) or STZ (1-3000 μg/mL) for 48 h at 37°C under a humidified atmosphere containing 5% CO2. Cells incubated in complete media including dWater in a final concentration of 0.1% served as control for solvent toxicity and cells incubated in complete medium are used as a control for the experiments. The effects of the tested drugs on tumor cell growth or viability are determined employing the MTT assay in accordance with the manufacturer's instructions. The IC50values (drug concentration that induces 50% inhibition of the cell growth) are calculated using the GraphPad Prism 4 program[2].

Synonyms	STZ, NSC-85998, U 9889, Streptozotocin
Molecular Weight	265.22
Formula	C8H15N3O7
CAS No.	18883-66-4

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

H2O: 113.3 mg/mL (427.19 mM), Need ultrasonic and warming

DMSO: 30 mg/mL (113.11 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Bolzán AD, et al. Mutat Res. 2002, 512(2-3), 121-13 2. Saini KS, et al. Biochem Mol Biol Int, 1996, 39(6), 1229-1236. 3. Arison RN, et al. Diabetes, 1967, 16(1), 51-56. 4. Iwase M, et al. Tohoku J Exp Med, 1989, 159(2), 83-90. 6. Acer S, et al. Oxidative stress of crystalline lens in rat menopausal model. Arq Bras Oftalmol. 2016 Jul-Aug;79(4):222-5.

Dose Conversion

You can also refer to dose conversion for different animals. More

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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Streptozocin 18883-66-4 Autophagy Cell Cycle/Checkpoint DNA Damage/DNA Repair Microbiology/Virology DNA Alkylator/Crosslinker DNA Alkylation DNA/RNA Synthesis Antibacterial Antibiotic U-9889 STZ inhibit Bacterial NSC-85998 U9889 Inhibitor U 9889 Streptozotocin inhibitor

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