Powder: -20°C for 3 years | In solvent: -80°C for 1 year
XMD17-109 (ERK5-IN-1) is a new selective ERK-5 inhibitor (EC50: 4.2 uM, HEK293 cells).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 35.00 | |
2 mg | In stock | $ 48.00 | |
5 mg | In stock | $ 79.00 | |
10 mg | In stock | $ 147.00 | |
25 mg | In stock | $ 233.00 | |
50 mg | In stock | $ 377.00 | |
100 mg | In stock | $ 567.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 112.00 |
Description | XMD17-109 (ERK5-IN-1) is a new selective ERK-5 inhibitor (EC50: 4.2 uM, HEK293 cells). |
Targets&IC50 | ERK5:162 nM(EC50) |
Cell Research | HeLa cells are maintained in DMEM supplemented with 10% FBS, 2 mM?l-glutamine, 50 U/mL penicillin G, and 50 μg/mL streptomycin. Before use HeLa cells are serum starved for 16 h in DMEM supplemented with 2 mM?l-glutamine, 50 U/mL penicillin G, and 50 μg/mL streptomycin. HeLa cells are then incubated with ERK5-IN-1 at the indicated concentrations for 1 h prior to stimulation with 0.5mol/Lsorbitol for 30 min. Cells are lysed in Triton lysis buffer (50 mM Tris-HCl, pH 7.5, 1 mM EGTA, 1 mM EDTA, 1 mM sodium orthovanadate, 50 mM sodium fluoride, 1 mM sodium pyrophosphate, 0.27mol/Lsucrose, 1 μM microcystin-LR, 1% (v/v) Triton X-100, 0.1% (v/v) 2-mercaptoethanol) and 20 μg of protein loaded per well. Samples are run on 8% polyacrylamide gels using standard methods. Proteins are transferred onto nitrocellulose membranes and specific proteins detected by immunoblotting. |
Synonyms | XMD17 109, ERK5-IN-1 |
Molecular Weight | 638.8 |
Formula | C36H46N8O3 |
CAS No. | 1435488-37-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 93 mg/mL (145.6 mM)
Ethanol: 93 mg/mL (145.6 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
XMD17-109 1435488-37-1 MAPK ERK Inhibitor inhibit XMD17109 XMD17 109 Extracellular signal regulated kinases ERK5-IN-1 XMD-17-109 inhibitor