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ANA-12

🥰Excellent
Catalog No. T2359Cas No. 219766-25-3
Alias ANA12, ANA 12

ANA-12 is a potent and selective TrkB antagonist that exhibits anxiolytic and antidepressant activity in mice.

ANA-12

ANA-12

🥰Excellent
Purity: 99.87%
Catalog No. T2359Alias ANA12, ANA 12Cas No. 219766-25-3
ANA-12 is a potent and selective TrkB antagonist that exhibits anxiolytic and antidepressant activity in mice.
Pack SizePriceAvailabilityQuantity
1 mg32 â‚¬In Stock
2 mg44 â‚¬In Stock
5 mg68 â‚¬In Stock
10 mg115 â‚¬In Stock
25 mg190 â‚¬In Stock
50 mg353 â‚¬In Stock
100 mg521 â‚¬In Stock
500 mg1.111 â‚¬In Stock
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Purity:99.87%
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Product Introduction

Bioactivity
Description
ANA-12 is a potent and selective TrkB antagonist that exhibits anxiolytic and antidepressant activity in mice.
Targets&IC50
TrkB:10 nM(Kd)
In vitro
In male Sprague-Dawley rats, ANA-12 (3 μg/dose) inhibits the medial nucleus of the solitary tract (mNTS) BDNF's effect on reducing food intake. In male C57BL/6 mice, ANA-12 (0.5 mg/kg, intraperitoneally) demonstrates antidepressant effects against lipopolysaccharide-induced depressive-like behavior. In wild-type male mice, ANA-12 reverses ethanol consumption and induces D3 receptor downregulation; however, it is ineffective in D3R -/- mice.
In vivo
In nnr5 PC12-TrkB cells, ANA-12 inhibits neurite outgrowth induced by brain-derived neurotrophic factor (BDNF) at concentrations as low as 10 nM. It selectively binds directly to the TrkB receptor, suppressing the downstream TrkB pathway without altering the functions of TrkA and TrkC. Furthermore, ANA-12 nullifies the effect of BDNF on increasing the inward current in DRG neurons.
Kinase Assay
ANA-12 Binding assay: Maxisorp ELISA 96-well plates are coated with various concentrations of Trk BECD -Fc, 20 mg/ml BSA, or 1 mg/mL IgG-Fc (polyclonal anti-TrkB) in a carbonate buffer (pH 9.6) overnight at 4°C. Plates are saturated with 0.5% BSA in PBS for 2 hours at room temperature and extensively washed in PBS-Tween 0.05%. Bodipy–ANA-12 is then incubated in 0.5% PBS-BSA for 1 hour at room temperature before the addition of BDNF in 0.5% PBS-BSA for another hour. After extensive washes in PBS-Tween 0.05%, the amount of bodipy–ANA-12 bound is quantified by fluorescence at 520 ± 10 nm. Detectability range for extrapolation analysis is assessed by coating ELISA plates with bodipy–ANA-12 and reading fluorescence at 520 ± 10 nm.
Cell Research
Modulation of neurite outgrowth by molecules is assessed in nnr5 PC12–TrkB, –TrkA, and –TrkC cells after addition of BDNF (1 nM), NGF (2 nM), and NT-3 (10 nM), respectively. The number of cells bearing neurites longer than 2 cells in diameter in each counting field is microscopically determined (2 fields per well, 3 wells per condition). Counting is performed blind each 24 hours for 3 days. (Only for Reference)
AliasANA12, ANA 12
Chemical Properties
Molecular Weight407.49
FormulaC22H21N3O3S
Cas No.219766-25-3
SmilesO=C(Nc1ccccc1C(=O)NC1CCCCNC1=O)c1cc2ccccc2s1
Relative Density.1.36 g/cm3 (Predicted)
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 4.07 mg/mL (10 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.4540 mL12.2702 mL24.5405 mL122.7024 mL
5 mM0.4908 mL2.4540 mL4.9081 mL24.5405 mL
10 mM0.2454 mL1.2270 mL2.4540 mL12.2702 mL

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