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ITE

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Purity:98%

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COA HNMR

ITE

Catalog No. T7202Cas No. 448906-42-1

ITE is a potent endogenous agonist of aryl hydrocarbon receptor (AhR) (Ki : 3 nM), has immunosuppressive activity.

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Pack Size	Price	Availability
2 mg	$34	In Stock
5 mg	$55	In Stock
10 mg	$97	In Stock
25 mg	$178	In Stock
50 mg	$313	In Stock
100 mg	$497	In Stock
1 mL x 10 mM (in DMSO)	$84	In Stock

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Product Introduction

Bioactivity

Description	ITE is a potent endogenous agonist of aryl hydrocarbon receptor (AhR) (Ki : 3 nM), has immunosuppressive activity.
In vitro	ITE dose- and time-dependently inhibited proliferation of HPAECs with a maximum inhibition of 83% at 20 μM after 6 days of treatment. ITE rapidly decreased AhR protein levels, while it increased mRNA levels of cytochrome P450 (CYP), family 1, member A1 (CYP1A1) and B1 (CYP1B1), indicating activation of the AhR/CYP1A1 and AhR/CYP1B1 pathways in HPAECs. The AhR siRNA significantly suppressed AhR protein expression, whereas it did not significantly alter ITE-inhibited growth of HPAECs[1].
In vivo	ITE diminishes colitis pathology through induction of Tregs; reduces inflammatory cytokines, inflammation score, and macrophage frequency; and induces DCs resulting in amelioration of colitis. Therefore, nontoxic endogenous ITE promotes the induction of Tregs and may be useful for the treatment of IBD[2].
Cell Research	Immunohistochemistry was performed to localize AhR expression in human lung tissues. The crystal violet method and MTT assay were used to determine ITE's effects on growth of HPAECs. The AhR activation in HPAECs was confirmed using Western blotting and RT-qPCR. The role of AhR in ITE-affected proliferation of HPAECs was assessed using siRNA knockdown method followed by the crystal violet method[1]
Animal Research	At the start of DSS induction, mice received 100 μl by intraperitoneal injection of vehicle and ITE (10 mg/kg body wt) twice a week on each Monday and Thursday until week 6 at the end point of the experiment. During a pilot study, we used several (5, 10, 20, 40, and 80 mg/kg body wt) doses of ITE and noticed that the 10-mg/kg dose was the lowest dose giving maximum protection. Therefore, Used this dose in entire study. At the experimental end point blood was collected by tail-vein bleedings and serum was obtained following centrifugation. For comparison, a similar treatment was also given to normal BL/6 mice to see the effect of ITE alone[2].

Chemical Properties

Molecular Weight	286.31
Formula	C14H10N2O3S
Cas No.	448906-42-1

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 41 mg/mL (143.20 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.4927 mL	17.4636 mL	34.9272 mL	174.6359 mL
5 mM	0.6985 mL	3.4927 mL	6.9854 mL	34.9272 mL
10 mM	0.3493 mL	1.7464 mL	3.4927 mL	17.4636 mL
20 mM	0.1746 mL	0.8732 mL	1.7464 mL	8.7318 mL
50 mM	0.0699 mL	0.3493 mL	0.6985 mL	3.4927 mL
100 mM	0.0349 mL	0.1746 mL	0.3493 mL	1.7464 mL

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