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Nitro blue tetrazolium chloride

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Purity:98.93%

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COA HNMR HPLC

Nitro blue tetrazolium chloride

Catalog No. T19032Cas No. 298-83-9

Nitro blue tetrazolium chloride (NBT) is a NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 M). A well-known scavenger of superoxide anions.Nitro blue tetrazolium chloride, a substrate for dehydrogenases, is used with the alkaline phosphatase substrate BCIP in western blotting and immunohistological staining procedures.

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Pack Size	Price	Availability	Quantity
500 mg	$42	In Stock
1 mL x 10 mM (in DMSO)	$30	In Stock

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Product Introduction

Bioactivity

Description	Nitro blue tetrazolium chloride (NBT) is a NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 M). A well-known scavenger of superoxide anions.Nitro blue tetrazolium chloride, a substrate for dehydrogenases, is used with the alkaline phosphatase substrate BCIP in western blotting and immunohistological staining procedures.
Alias	NBT

Chemical Properties

Molecular Weight	817.64
Formula	C40H30Cl2N10O6
Cas No.	298-83-9

Storage & Solubility Information

Storage

keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 33.33 mg/mL (40.76 mM)

70%DMF: 100 mg/mL (122.30 mM)

Solution Preparation Table

70%DMF/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.2230 mL	6.1152 mL	12.2303 mL	61.1516 mL
5 mM	0.2446 mL	1.2230 mL	2.4461 mL	12.2303 mL
10 mM	0.1223 mL	0.6115 mL	1.2230 mL	6.1152 mL
20 mM	0.0612 mL	0.3058 mL	0.6115 mL	3.0576 mL

70%DMF

	1mg	5mg	10mg	50mg
50 mM	0.0245 mL	0.1223 mL	0.2446 mL	1.2230 mL
100 mM	0.0122 mL	0.0612 mL	0.1223 mL	0.6115 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

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A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

Dose Conversion

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