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NU1025 (NSC-696807) is a potent PARP inhibitor with IC50 of 400 nM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
2 mg | 38 € | In Stock | |
5 mg | 61 € | In Stock | |
10 mg | 99 € | In Stock | |
25 mg | 188 € | In Stock | |
50 mg | 318 € | In Stock | |
100 mg | 501 € | In Stock | |
500 mg | 1.121 € | In Stock | |
1 mL x 10 mM (in DMSO) | 61 € | In Stock |
Description | NU1025 (NSC-696807) is a potent PARP inhibitor with IC50 of 400 nM. |
Targets&IC50 | PARP:400 nM |
In vitro | NU1025 (0.2 mM) treatment attenuates Water2 induced cytotoxicity. NU1025 per se does not have any effect on cell viability. NU1025 pretreatment significantly increases cell viability (82.59 4.67%) in SIN-1 (0.8 mM) exposed cells.[2] NU1025 has no detectable effect on the proliferation of D54 and U251 cells. Treatment with NU1025 markedly inhibits the enhanced activation of PARP-1 induced by TPT and RT treatment.[3] No DNA strand breakage is detected following exposure to 200 μM NU1025 alone.[4] |
In vivo | Treatment with NU1025 (1 and 3 mg/kg) reduces the infarction to 25% and 45% versus vehicle treated rats, respectively. NU1025 (1 and 3 mg/kg) treatment significantly reduces edema volume. NU1025 also produces significant improvement in neurological deficits.[2] |
Kinase Assay | PARP activation assay: Cells are suspended in hypotonic buffer (9 mM HEPES, pH 7.8, 4.5% (v/v) dextran, 4.5 mM MgCl2 and 5 mM DTT) at 1.5 × 107/mL on ice for 30 min, then 9 vol of isotonic buffer (40 mM HEPES, pH 7.8, 130 mM KCl, 4% (v/v) dextran, 2 mM EGTA, 2.3 mM MgCl2, 225 mM sucrose and 2.5 mM DTT) is added. The reaction is started by adding 300 μL cells to 100 μL 300 μM NAD+ containing [32P]-NAD+, and terminated by the addition of 2 mL ice-cold 10% (w/v) TCA +10% (w/v) sodium pyrophosphate. After 30 min on ice the precipitated 32P-labelled ADP-ribose polymers are filtered, washed five times with 1% (v/v) TCA, 1% (v/v) sodium pyrophosphate, dried and counted. |
Cell Research | Cells are seeded in 96-well plates at a density of 2,500 cells/well and treated with the indicated doses of NU1025. Adherent cells are irradiated in medium with 250 kVp X-rays (dose rate 0.5 Gy/min). Untreated cells are used as a control. Following an up to 5 day incubation, cell proliferation is assessed by MTT assay.(Only for Reference) |
Alias | NSC 696807 |
Molecular Weight | 176.17 |
Formula | C9H8N2O2 |
Cas No. | 90417-38-2 |
Smiles | Cc1nc2c(O)cccc2c(=O)[nH]1 |
Relative Density. | 1.42 g/cm3 |
Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | |||||||||||||||||||||||||||||||||||
Solubility Information | DMSO: 40 mg/mL (227.05 mM), Sonication is recommended. 1eq. NaOH: 17.6 mg/mL (100 mM) | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
1eq. NaOH/DMSO
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