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NU1025

🥰Excellent
Catalog No. T6912Cas No. 90417-38-2
Alias NSC 696807

NU1025 (NSC-696807) is a potent PARP inhibitor with IC50 of 400 nM.

NU1025

NU1025

🥰Excellent
Purity: 98.00%
Catalog No. T6912Alias NSC 696807Cas No. 90417-38-2
NU1025 (NSC-696807) is a potent PARP inhibitor with IC50 of 400 nM.
Pack SizePriceAvailabilityQuantity
2 mg38 â‚¬In Stock
5 mg61 â‚¬In Stock
10 mg99 â‚¬In Stock
25 mg188 â‚¬In Stock
50 mg318 â‚¬In Stock
100 mg501 â‚¬In Stock
500 mg1.121 â‚¬In Stock
1 mL x 10 mM (in DMSO)61 â‚¬In Stock
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Purity:98.00%
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Product Introduction

Bioactivity
Description
NU1025 (NSC-696807) is a potent PARP inhibitor with IC50 of 400 nM.
Targets&IC50
PARP:400 nM
In vitro
NU1025 (0.2 mM) treatment attenuates Water2 induced cytotoxicity. NU1025 per se does not have any effect on cell viability. NU1025 pretreatment significantly increases cell viability (82.59 4.67%) in SIN-1 (0.8 mM) exposed cells.[2] NU1025 has no detectable effect on the proliferation of D54 and U251 cells. Treatment with NU1025 markedly inhibits the enhanced activation of PARP-1 induced by TPT and RT treatment.[3] No DNA strand breakage is detected following exposure to 200 μM NU1025 alone.[4]
In vivo
Treatment with NU1025 (1 and 3 mg/kg) reduces the infarction to 25% and 45% versus vehicle treated rats, respectively. NU1025 (1 and 3 mg/kg) treatment significantly reduces edema volume. NU1025 also produces significant improvement in neurological deficits.[2]
Kinase Assay
PARP activation assay: Cells are suspended in hypotonic buffer (9 mM HEPES, pH 7.8, 4.5% (v/v) dextran, 4.5 mM MgCl2 and 5 mM DTT) at 1.5 × 107/mL on ice for 30 min, then 9 vol of isotonic buffer (40 mM HEPES, pH 7.8, 130 mM KCl, 4% (v/v) dextran, 2 mM EGTA, 2.3 mM MgCl2, 225 mM sucrose and 2.5 mM DTT) is added. The reaction is started by adding 300 μL cells to 100 μL 300 μM NAD+ containing [32P]-NAD+, and terminated by the addition of 2 mL ice-cold 10% (w/v) TCA +10% (w/v) sodium pyrophosphate. After 30 min on ice the precipitated 32P-labelled ADP-ribose polymers are filtered, washed five times with 1% (v/v) TCA, 1% (v/v) sodium pyrophosphate, dried and counted.
Cell Research
Cells are seeded in 96-well plates at a density of 2,500 cells/well and treated with the indicated doses of NU1025. Adherent cells are irradiated in medium with 250 kVp X-rays (dose rate 0.5 Gy/min). Untreated cells are used as a control. Following an up to 5 day incubation, cell proliferation is assessed by MTT assay.(Only for Reference)
AliasNSC 696807
Chemical Properties
Molecular Weight176.17
FormulaC9H8N2O2
Cas No.90417-38-2
SmilesCc1nc2c(O)cccc2c(=O)[nH]1
Relative Density.1.42 g/cm3
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 40 mg/mL (227.05 mM), Sonication is recommended.
1eq. NaOH: 17.6 mg/mL (100 mM)
Solution Preparation Table
1eq. NaOH/DMSO
1mg5mg10mg50mg
1 mM5.6763 mL28.3817 mL56.7634 mL283.8168 mL
5 mM1.1353 mL5.6763 mL11.3527 mL56.7634 mL
10 mM0.5676 mL2.8382 mL5.6763 mL28.3817 mL
20 mM0.2838 mL1.4191 mL2.8382 mL14.1908 mL
50 mM0.1135 mL0.5676 mL1.1353 mL5.6763 mL
100 mM0.0568 mL0.2838 mL0.5676 mL2.8382 mL

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