Oxythiamine (Hydroxythiamin) is an antivitamin of thiamine and a transketolase (TK) inhibitor. Oxythiamine inhibits cancer cell proliferation and induces cell cycle arrest. Oxythiamine has potential anticancer activity, inhibits non-oxidative synthesis of ribose, and induces apoptosis.

MIA PaCa-2 cells:14.95 μM

Oxythiamine for MIA PaCa-2 cells 5-320 μM for 2 days IC50 is 14.95 μM MTT, MIA PaCa-2 cells concentrations of Oxythiamine (0 μM, 5 μM, 50 μM and 500 μM) for 48 hours two-dimensional gel electrophoresis (2DE). Oxythiamine altered protein expression in a dose dependent manner.[1]
The MIC and MFC values of Oxythiamine were in the range 0.08 × 103 to 10 × 103 mg/L. [2]
A549 cells were co-cultured with Oxythiamine (from 0.1 to 100 μM) for 6–12 h CCK-8 assay
Oxythiamine contributes to a significant reduction of A549 cell viability of 11.7%, 23.6%, and 28.2% at 12, 24, and 48 h. [3]
Oxythiamine arrests the cell growth in G1 phase of cell cycle. [3]
Oxythiamine inhibits the growth of A549 cells by initially slowing-down cell cycle and by leading to apoptosis. [3]

s.c. injection C57BL/6 mice with LLC cells and supplemented the mice with a low- or a high-dose of Oxythiamine (250 or 500 mg/kg BW) daily for 5 wk. During the 5-wk period, Oxythiamine supplementation decreased plasma MMP-2 activity in a dose-dependent manner, and this effect was significant after 4 wk of tumor cell implantation. [4]