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STF-083010

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Purity:98.85%

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COA HNMR HPLC

STF-083010

Catalog No. T6681Cas No. 307543-71-1

STF-083010 is a selective inhibitor of the IRE1α endonuclease.

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Pack Size	Price	Availability
5 mg	$47	In Stock
10 mg	$73	In Stock
25 mg	$123	In Stock
50 mg	$215	In Stock
100 mg	$387	In Stock
200 mg	$585	In Stock
500 mg	$853	In Stock
1 mL x 10 mM (in DMSO)	$53	In Stock

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Product Introduction

Bioactivity

Description	STF-083010 is a selective inhibitor of the IRE1α endonuclease.
In vitro	WaC3 cells respond to treatments with STF-083010 with gradually decreased growth. In MEC1 and MEC2 cells, STF-083010 produces 20% growth inhibition in 48 hours cultures. In MM.1R MM, MM.1S, and RPMI 8226 cell lines, STF-083010 displays cytostatic and cytotoxic activity in a dose and time-dependent manner. In Eμ-TCL1 CLL cells, STF-083010 displays about 70% growth inhibition after 3 days culture. In SU8686, Panc0403, and MiaPaCa2 cell lines, STF-083010 blocks IRE1α's endonuclease and inhibits XBP1 splicing activity without affecting its kinase activity.
In vivo	STF-083010 (i.p., 30 mg/kg) signi?cantly inhibits the growth of the tumor in human multiple myeloma (MM) xenografts model.
Kinase Assay	Autophosphorylation activity is measured by adding of 32P-γ ATP. Endonuclease activity is measured by the adding of radiolabeled HAC1 508-nt RNA substrate synthesized in vitro using α32P-UTP. Mix STF083010 with recombinant hIRE1 protein, radiolabeled HAC1 508 nt RNA, and appropriate buffers to incubate. Kinase activity is quantitated by polyacrylamide gel electrophoresis. RNAsecleavage products are quantitated by 32P-γATP or 32P-UTP autoradiography.
Cell Research	3×10E3 (Panc0403, Panc1005, BxPc3, and MiaPaCa2) cell lines are seeded in 96-well plates overnight, and STF-083010(~50 μM) treatment for 48 hours started the next day.
Animal Research	NOD/SCID/IL2Rγ null mice were injected intraperitoneally of STF-083010(30 mg/kg) dissolved in 16% chremophor.
Alias	STF083010

Chemical Properties

Molecular Weight	317.38
Formula	C15H11NO3S2
Cas No.	307543-71-1

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 40 mg/mL (126.03 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.1508 mL	15.7540 mL	31.5080 mL	157.5399 mL
5 mM	0.6302 mL	3.1508 mL	6.3016 mL	31.5080 mL
10 mM	0.3151 mL	1.5754 mL	3.1508 mL	15.7540 mL
20 mM	0.1575 mL	0.7877 mL	1.5754 mL	7.8770 mL
50 mM	0.0630 mL	0.3151 mL	0.6302 mL	3.1508 mL
100 mM	0.0315 mL	0.1575 mL	0.3151 mL	1.5754 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

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DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

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Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

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Tech Support

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