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BAM7

BAM7
BAM 7 is a direct and specific activator of proapoptotic Bax (EC50: 3.3 μM).
Catalog No. T2453Cas No. 331244-89-4
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Purity:99.23%
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BAM7

Catalog No. T2453Cas No. 331244-89-4
BAM 7 is a direct and specific activator of proapoptotic Bax (EC50: 3.3 μM).
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
5 mg$44In Stock
10 mg$62In Stock
25 mg$130In Stock
50 mg$237In Stock
100 mg$356In Stock
200 mg$497In Stock
1 mL x 10 mM (in DMSO)$48In Stock
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Product Introduction

Bioactivity
Description
BAM 7 is a direct and specific activator of proapoptotic Bax (EC50: 3.3 μM).
Targets&IC50
Bax:3.3 mM(EC50)
In vivo
In vitro experiments show that BAM7 induces BAX-mediated pore formation, BAX oligomerization, and BAX-dependent cell death. By activating BAX within cells, BAM7 selectively triggers BAX-mediated apoptosis. Its efficacy is limited to cells containing BAX, where it induces the biochemical and morphological hallmarks of BAX-mediated apoptosis. BAM7 binds directly to the N-terminal BH3 domain of BAX, facilitating interaction at the surface and BAX activation via the BIM BH3 helix. Moreover, BAM7 dose-dependently and temporally triggers the conversion of BAX from monomers to oligomers.
Kinase Assay
Fluorescence polarization binding assays: Direct binding curves are first generated by incubating FITC-BIM SAHB (50 nM) with serial dilutions of fulllength BAX, BCL-XLΔC, MCL-1ΔNΔC, BFL-1/A1ΔC or BAKΔC and fluorescence polarization measured at 20 minutes on a SpectraMax M5 microplate reader. For competition assays, a serial dilution of small molecule or acetylated BIM SAHB (Ac-BIM SAHB) is combined with FITC-BIM SAHB (50 nM), followed by the addition of recombinant protein at ~EC75 concentration, as determined by the direct binding assay (BAX, BAKΔC: 500 nM; BCL-XLΔC, MCL-1ΔNΔC, BFL-1/A1ΔC: 200 nM). Fluorescence polarization is measured at 20 minutes and IC50 values calculated by nonlinear regression analysis of competitive binding curves using Prism software.
Cell Research
MEFs (2.5 × 103 cells per well) are seeded in 96-well opaque plates for 18-24 h and then incubated with serial dilutions of BAM7, ANA-BAM16 or vehicle (0.15% (v/v) DMSO) in DMEM at 37 °C in a final volume of 100 μL. Cell viability is assayed at 24 h by addition of CellTiter-Glo reagent according to the manufacturer's protocol, and luminescence is measured using a SpectraMax M5 microplate reader. Viability assays are performed in at least triplicate, and the data are normalized to vehicle-treated control wells.(Only for Reference)
Chemical Properties
Molecular Weight405.47
FormulaC21H19N5O2S
Cas No.331244-89-4
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 4.1 mg/mL (10 mM)
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.4663 mL12.3314 mL24.6627 mL123.3137 mL
5 mM0.4933 mL2.4663 mL4.9325 mL24.6627 mL
10 mM0.2466 mL1.2331 mL2.4663 mL12.3314 mL

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