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Berzosertib

Catalog No. T2669   CAS 1232416-25-9
Synonyms: VX-970, VE-822

Berzosertib (VE-822) has been used in trials studying the treatment of Ovarian Neoplasms, Ovarian Serous Tumor, Adult Solid Neoplasm, Advanced Solid Tumor, and Advanced Solid Neoplasm, among others.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Berzosertib Chemical Structure
Berzosertib, CAS 1232416-25-9
Pack Size Availability Price/USD Quantity
1 mg In stock $ 33.00
2 mg In stock $ 45.00
5 mg In stock $ 70.00
10 mg In stock $ 100.00
25 mg In stock $ 228.00
50 mg In stock $ 343.00
100 mg In stock $ 538.00
200 mg In stock $ 665.00
500 mg In stock $ 1,050.00
1 mL * 10 mM (in DMSO) In stock $ 70.00
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Purity: 100%
Purity: 99.7%
Purity: 99.19%
Purity: 99.19%
Purity: 99.19%
Purity: 97.34%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Berzosertib (VE-822) has been used in trials studying the treatment of Ovarian Neoplasms, Ovarian Serous Tumor, Adult Solid Neoplasm, Advanced Solid Tumor, and Advanced Solid Neoplasm, among others.
Targets&IC50 ATR:19 nM
Kinase Assay A375 cells are pre-treated with 1 μM JNK-IN-8 for the indicated amounts of time. Remove the medium and wash 3 times with PBS. Resuspend the cell pellet with 1 mL Lysis Buffer (1% NP-40, 1% CHAPS, 25 mM Tris, 150 mM NaCl, Phosphatase Inhibitor Cocktail, and Protease Inhibitor Cocktail). Rotate end-to-end for 30 min at 4°C. Lysates are cleared by centrifugation at 14000 rpm for 15 min in the Eppendorf. The cleared lysates gel filtered into Kinase Buffer (0.1% NP-40, 20 mM HEPES, 150 mM NaCl, Phosphatase Inhibitor Cocktail, Protease Inhibitor Cocktail) using Bio-Rad 10DG colums. The total protein concentration of the gel-filtered lysate should be around 5-15 mg/mL. Cell lysate is labeled with the probe from ActivX at 5 μM for 1 hour. Samples are reduced with DTT, and cysteines are blocked with iodoacetamide and gel filtered to remove excess reagents and exchange the buffer. Add 1 volume of 2X Binding Buffer (2% Triton-100, 1% NP-40, 2 mM EDTA, 2X PBS) and 50 μL streptavidin bead slurry and rotate end-to-end for 2 hours, centrifuge at 7000 rpm for 2 min. Wash 3 times with 1X Binding Buffer and 3 times with PBS. Add 30 μL 1X sample buffer to beads, heat samples at 95°C for 10 min. Run samples on an SDS-PAGE gel at 110V. After transferred, the membrane is immunoblotted with JNK antibody[1].
Cell Research VE-822 is dissolved in DMSO and stored, and then diluted with appropriate media before use[1]. Gemcitabine (10 nM) is added 24 h pre-XRT and is replaced with fresh medium before addition of VE-822. PSN-1 cells are treated with VE-822 (80 nM) for 1 h before, through to 18 h after, XRT (6 Gy). Apoptosis is analyzed 48 h after XRT by flow cytometry using an Annexin V-FITC kit with PI[1].
Synonyms VX-970, VE-822
Molecular Weight 463.55
Formula C24H25N5O3S
CAS No. 1232416-25-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 34 mg/mL (73.3 mM)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

TargetMolReferences and Literature

1. Fokas E, et al. Cell Death Dis, 2012, 3, e441. 2. Fokas E, et al. Cancer Treat Rev, 2013, pii: S0305-7372(13)00065-0. 3. Konstantinopoulos P A , Cheng S C , Hendrickson A E W , et al. Berzosertib plus gemcitabine versus gemcitabine alone in platinum-resistant high-grade serous ovarian cancer: a multicentre, open-label, randomised, phase 2 trial[J]. The Lancet Oncology, 2020, 21(7).

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Clinical Compound Library Anti-Cancer Active Compound Library Anti-Cancer Drug Library Highly Selective Inhibitor Library Anti-Cancer Compound Library Neuronal Differentiation Compound Library ReFRAME Related Library Glycolysis Compound Library Anti-Liver Cancer Compound Library Reprogramming Compound Library

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Keywords

Berzosertib 1232416-25-9 DNA Damage/DNA Repair PI3K/Akt/mTOR signaling ATM/ATR VE 822 VX 970 VX-970 VX970 VE822 VE-822 inhibitor inhibit

 

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