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FCCP

Catalog No. T6834 CAS 370-86-5

Synonyms: Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, Trifluoromethoxy carbonylcyanide phenylhydrazone

FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone) is an oxidative phosphorylation (OXPHOS) inhibitor and mitochondrial proton carrier uncoupler. FCCP is often used as an apoptosis inducer.

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FCCP, CAS 370-86-5

Pack Size	Availability	Price/USD
5 mg	In stock	$ 36.00
10 mg	In stock	$ 51.00
25 mg	In stock	$ 92.00
50 mg	In stock	$ 166.00
100 mg	In stock	$ 310.00
200 mg	In stock	$ 518.00
500 mg	In stock	$ 828.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 99.5%

Purity: 99.44%

Purity: 98%

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Description	FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone) is an oxidative phosphorylation (OXPHOS) inhibitor and mitochondrial proton carrier uncoupler. FCCP is often used as an apoptosis inducer.
In vitro	METHODS: Rat ventricular myocytes were treated with FCCP (10-1000 nM) for 1400 sec, and cellular oxygen consumption was measured using a Clark Oxygen Electrode. RESULTS: Oxygen consumption increased significantly, immediately, and in a dose-dependent manner after the addition of FCCP. [1] METHODS: Rat adrenal medullary chromaffinoma cells PC12 were treated with FCCP (30 µM) for 0.5-2 h. The rate of protein synthesis was measured using [3H]methionine. RESULTS: FCCP treatment produced a strong inhibition (68%) of protein synthesis rate for at least 2 hours. [2]
In vivo	METHODS: To test the effect on stroke, FCCP (1 mg/kg) was injected intraperitoneally into a C57BL/6J mouse model of stroke, followed by one hour of transient middle cerebral artery occlusion (tMCAO). RESULTS: Infarct volumes in the cortex, striatum, and whole hemisphere were significantly increased in mice pretreated with FCCP. Mice receiving FCCP had significantly increased neurologic deficit scores compared to carriers. [3] METHODS: To assay anti-tumor activity in vivo, FCCP (1 mg/kg) and cisplatin (2 mg/kg) were intraperitoneally injected every two days for two weeks into C57BL/6 mice harboring mouse ovarian epithelial carcinoma tumor ID8. RESULTS: The combination of FCCP and cisplatin inhibited tumor growth via OMA1-induced mitochondrial and ER stress. [4]
Cell Research	Protein synthesis rate is assayed in 24-mm diameter multi-well dishes with fresh medium containing 0.175 Ci/mmol of [3H]methionine (200 μM), for 30 min at 37°C. PC12 cells are treated with FCCP for different period of times. (Only for Reference)

Synonyms	Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, Trifluoromethoxy carbonylcyanide phenylhydrazone
Molecular Weight	254.17
Formula	C10H5F3N4O
CAS No.	370-86-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 25.4 mg/mL (100 mM)

References and Literature

1. Brennan JP, et al. FCCP is cardioprotective at concentrations that cause mitochondrial oxidation without detectable depolarisation. Cardiovasc Res. 2006 Nov 1;72(2):322-30. 2. Muñoz F, et al. Carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) induces initiation factor 2 alpha phosphorylation and translation inhibition in PC12 cells. FEBS Lett. 2001 Mar 9;492(1-2):156-9. 3. Grasmick KA, et al. Uncoupling of the Electron Transport Chain Compromises Mitochondrial Oxidative Phosphorylation and Exacerbates Stroke Outcomes. J Neuroinfect Dis. 2018;9(4):283. 4. Cheng M, et al. The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer. Int J Mol Sci. 2022 Jan 25;23(3):1320.

Citations

1. Liu H, Sun Y, Xu H, et al. PTEN-induced putative kinase 1 regulates mitochondrial quality control and is essential for the maturation of human induced pluripotent stem cell-derived cardiomyocytes. Genes & Diseases. 2022 2. Liang S, Zhu C, Suo C, et al. Mitochondrion-Localized SND1 Promotes Mitophagy and Liver Cancer Progression Through PGAM5. Frontiers in Oncology. 2022, 12: 857968-857968 3. Shi J, Zhang F, Chen L, et al. Systemic mitochondrial disruption is a key event in the toxicity of bacterial pore‐forming toxins to Caenorhabditis elegans. Environmental Microbiology. 2021 Sep;23(9):4896-4907

Related compound libraries

This product is contained In the following compound libraries：

Anti-Aging Compound Library Mitochondria-Targeted Compound Library Target-Focused Phenotypic Screening Library Cuproptosis Compound Library Bioactive Compound Library Bioactive Compounds Library Max Ion Channel Inhibitor Library Anti-Cancer Compound Library Fluorochemical Library Metabolism Compound Library

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Keywords

FCCP 370-86-5 Membrane transporter/Ion channel Metabolism ATPase Mitochondrial Metabolism inhibit Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Inhibitor Trifluoromethoxy carbonylcyanide phenylhydrazone inhibitor

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