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FH535

Catalog No. T2413   CAS 108409-83-2

FH535, a Wnt/β-catenin signaling and PPAR inhibitor, exhibits anti-tumor activities.

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FH535 Chemical Structure
FH535, CAS 108409-83-2
Pack Size Availability Price/USD Quantity
5 mg In stock $ 32.00
10 mg In stock $ 52.00
25 mg In stock $ 95.00
50 mg In stock $ 167.00
100 mg In stock $ 288.00
200 mg In stock $ 425.00
500 mg In stock $ 688.00
1 mL * 10 mM (in DMSO) In stock $ 58.00
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Purity: 99.5%
Purity: 99.2%
Purity: 98.98%
Purity: 98.25%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description FH535, a Wnt/β-catenin signaling and PPAR inhibitor, exhibits anti-tumor activities.
Kinase Assay High-throughput Library Screen: Three copies of the optimized or mutated Tcf-binding element from TOPFLASH or FOPFLASH driving a secreted alkaline phosphatase reporter gene are cloned into pCEP4 plasmid, replacing the cytomegalovirus promoter. The plasmids are transfected into HepG2 cells, and hygromycin-resistant clones are pooled. Library screening is done at 20 μmol/L concentration in HepG2 serum-free media. Hits are tested in the HCT116 cell line for inhibition of TOPFLASH luciferase activity but not for inhibition of a reporter activity controlled from β-actin promoter.
Cell Research Cell viability is determined by the modified 3H-thymidine incorporation assay. Briefly, cells are plated in 96-well microplates for 24 h and treated in triplicate with various concentrations of the test compound. After 48 h of compound exposure, the cells are incubated for an additional 48 h in compound-free medium. The cells are then incubated in medium containing 3H-thymidine for 24 h, washed and mixed with the scintillant in the 96-well plate. Individual wells are counted with a 96-well scintillation counter and the LC50 is calculated.(Only for Reference)
Molecular Weight 361.2
Formula C13H10Cl2N2O4S
CAS No. 108409-83-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 27.1 mg/mL (75 mM)

TargetMolReferences and Literature

1. Handeli S, et al. Mol Cancer Ther. 2008, 7(3), 521-529. 2. Polk WW. Int J Toxicol. 2012, 31(4), 380-389. 3. Gedaly R, et al. PLoS One. 2014, 9(6), e99272.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Neurodegenerative Disease Compound Library Anti-Cancer Active Compound Library Bioactive Compounds Library Max Anti-Colorectal Cancer Compound Library Anti-Alzheimer's Disease Compound Library Transcription Factor-Targeted Compound Library Lipid Metabolism Compound Library NO PAINS Compound Library Glycometabolism Compound Library Anti-Aging Compound Library

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Keywords

FH535 108409-83-2 Cytoskeletal Signaling DNA Damage/DNA Repair Metabolism Stem Cells PPAR Wnt/beta-catenin Beta catenin FH 535 β-catenin inhibit FH-535 Wnt Inhibitor Peroxisome proliferator-activated receptors inhibitor

 

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