Powder: -20°C for 3 years | In solvent: -80°C for 1 year
FH535, a Wnt/β-catenin signaling and PPAR inhibitor, exhibits anti-tumor activities.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | In stock | $ 32.00 | |
10 mg | In stock | $ 52.00 | |
25 mg | In stock | $ 95.00 | |
50 mg | In stock | $ 167.00 | |
100 mg | In stock | $ 288.00 | |
200 mg | In stock | $ 425.00 | |
500 mg | In stock | $ 688.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 58.00 |
Description | FH535, a Wnt/β-catenin signaling and PPAR inhibitor, exhibits anti-tumor activities. |
Kinase Assay | High-throughput Library Screen: Three copies of the optimized or mutated Tcf-binding element from TOPFLASH or FOPFLASH driving a secreted alkaline phosphatase reporter gene are cloned into pCEP4 plasmid, replacing the cytomegalovirus promoter. The plasmids are transfected into HepG2 cells, and hygromycin-resistant clones are pooled. Library screening is done at 20 μmol/L concentration in HepG2 serum-free media. Hits are tested in the HCT116 cell line for inhibition of TOPFLASH luciferase activity but not for inhibition of a reporter activity controlled from β-actin promoter. |
Cell Research | Cell viability is determined by the modified 3H-thymidine incorporation assay. Briefly, cells are plated in 96-well microplates for 24 h and treated in triplicate with various concentrations of the test compound. After 48 h of compound exposure, the cells are incubated for an additional 48 h in compound-free medium. The cells are then incubated in medium containing 3H-thymidine for 24 h, washed and mixed with the scintillant in the 96-well plate. Individual wells are counted with a 96-well scintillation counter and the LC50 is calculated.(Only for Reference) |
Molecular Weight | 361.2 |
Formula | C13H10Cl2N2O4S |
CAS No. | 108409-83-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 27.1 mg/mL (75 mM)
You can also refer to dose conversion for different animals. More
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FH535 108409-83-2 Cytoskeletal Signaling DNA Damage/DNA Repair Metabolism Stem Cells PPAR Wnt/beta-catenin Beta catenin FH 535 β-catenin inhibit FH-535 Wnt Inhibitor Peroxisome proliferator-activated receptors inhibitor