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FH535

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Purity:99.5%

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COA HNMR LCMS

FH535

Catalog No. T2413Cas No. 108409-83-2

FH535, a Wnt/β-catenin signaling and PPAR inhibitor, exhibits anti-tumor activities.

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Pack Size	Price	Availability
5 mg	$32	In Stock
10 mg	$52	In Stock
25 mg	$95	In Stock
50 mg	$167	In Stock
100 mg	$288	In Stock
200 mg	$425	In Stock
500 mg	$688	In Stock
1 mL x 10 mM (in DMSO)	$58	In Stock

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Product Introduction

Bioactivity

Description	FH535, a Wnt/β-catenin signaling and PPAR inhibitor, exhibits anti-tumor activities.
In vivo	FH535 inhibits β-catenin/Tcf-mediated transcription and also suppresses the aggregation of co-activator GRIP1 and β-catenin with PPARδ and PPARγ. In cancer cells with elevated or active Wnt/β-catenin pathways, FH535 can inhibit cell proliferation.
Kinase Assay	High-throughput Library Screen: Three copies of the optimized or mutated Tcf-binding element from TOPFLASH or FOPFLASH driving a secreted alkaline phosphatase reporter gene are cloned into pCEP4 plasmid, replacing the cytomegalovirus promoter. The plasmids are transfected into HepG2 cells, and hygromycin-resistant clones are pooled. Library screening is done at 20 μmol/L concentration in HepG2 serum-free media. Hits are tested in the HCT116 cell line for inhibition of TOPFLASH luciferase activity but not for inhibition of a reporter activity controlled from β-actin promoter.
Cell Research	Cell viability is determined by the modified 3H-thymidine incorporation assay. Briefly, cells are plated in 96-well microplates for 24 h and treated in triplicate with various concentrations of the test compound. After 48 h of compound exposure, the cells are incubated for an additional 48 h in compound-free medium. The cells are then incubated in medium containing 3H-thymidine for 24 h, washed and mixed with the scintillant in the 96-well plate. Individual wells are counted with a 96-well scintillation counter and the LC50 is calculated.(Only for Reference)

Chemical Properties

Molecular Weight	361.2
Formula	C13H10Cl2N2O4S
Cas No.	108409-83-2

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 65 mg/mL (179.96 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7685 mL	13.8427 mL	27.6855 mL	138.4275 mL
5 mM	0.5537 mL	2.7685 mL	5.5371 mL	27.6855 mL
10 mM	0.2769 mL	1.3843 mL	2.7685 mL	13.8427 mL
20 mM	0.1384 mL	0.6921 mL	1.3843 mL	6.9214 mL
50 mM	0.0554 mL	0.2769 mL	0.5537 mL	2.7685 mL
100 mM	0.0277 mL	0.1384 mL	0.2769 mL	1.3843 mL

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Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

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Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

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mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

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