Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Kaempferol (Robigenin) is a natural flavonoid and an inverse agonist of ERRα and ERRγ. Kaempferol has a wide range of antitumor, anti-inflammatory, antioxidant, antibacterial and antiviral activities.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 mg | In stock | $ 46.00 | |
100 mg | In stock | $ 58.00 | |
200 mg | In stock | $ 91.00 | |
500 mg | In stock | $ 197.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 50.00 |
Description | Kaempferol (Robigenin) is a natural flavonoid and an inverse agonist of ERRα and ERRγ. Kaempferol has a wide range of antitumor, anti-inflammatory, antioxidant, antibacterial and antiviral activities. |
In vitro | Kaempferol, a natural flavonoid found extensively in fruits and vegetables, exhibits powerful anti-inflammatory properties by suppressing interleukin-4 and cyclo-oxygenase 2 expression through the inhibition of Src kinase activity and downregulation of the NFκB pathway. It effectively halts angiogenesis and promotes the apoptosis of ovarian cancer cells. Notably, long-term consumption of kaempferol has been associated with a significantly reduced risk of ovarian cancer among American female nurses, according to prospective studies. In tests on ovarian cancer cell lines, kaempferol demonstrated a significant, concentration-dependent reduction in cell proliferation following a 24-hour exposure, particularly at concentrations of 40 μM or higher. Besides its anti-cancer effects, kaempferol also markedly inhibits NADPH oxidase activity, reducing reactive oxygen species (ROS) by directly binding to NADPH oxidase, and protects against Ang II-induced sinus nodal cell death by reducing CAMKII oxidation. Additionally, in sensitized RBL-2H3 cells, kaempferol at doses of 10-20 μM suppresses the release of inflammatory agents and attenuates the activation of Syk and PLCγ, as well as reduces COX2 induction in response to allergen challenge, demonstrating its broad-spectrum biological activities. |
In vivo | COX2 induction was confirmed in the airways of BSA-challenged BALB/c mice, while untreated control mice displayed a lack of COX2. Inhalation of BSA led to an enhanced induction of COX2 (dark brown staining) in the mouse airway, which was reversed by the oral administration of Kaempferol. Additionally, marked goblet cell hyperplasia and epithelial thickening were observed in BSA-challenged mice; however, these symptoms were completely alleviated with the supplementation of 20 mg/kg Kaempferol[4]. |
Kinase Assay | Right atria or sinus nodal cells are homogenized in lysis buffer consisting of (50 mM Tris-HCl pH 7.5, 100 mM KCl, 1 mM ethylenediamine tetraacetic acid, 1 mM ethylene glycol tetraacetic acid, 1 mM dithiothreitol, 0.1 mM phenylmethylsulfonyl fluoride, 0.5 mM Benzamidine, 20 mg/L Leupeptin, 20 mM sodium pyrophosphate, 50 mM NaF, and 50 mM sodium β-glycerophosphate), and total protein content is determined by the Bradford assay. Caspase-3 activity is determined by EnzChek Caspase-3 Assay Kit[3]. |
Cell Research | Kaempferol is prepared in DMSO (100 mM) and stored (-20°C), and then diluted with appropriate medium[2]. Ovarian cancer cells are seeded in 96-well plates at 2000 cells/well and incubated overnight before treatment with 0-160 μM Kaempferol for 24 hours in triplicates. The medium is removed, and the plates are freeze-thawed to lyse cells. Each well is added with 200 μL 1× CyQUANT cell lysis buffer containing 5x SYBR Green I and incubated at room temperature (RT) for 5 minutes. The reaction (50 μL) is transferred to PCR strip tubes and the fluorescent signal is measured at 90°C with a real-time Chromo4 PCR instrument. To ensure that cell proliferation assays are performed within a linear range of cell numbers, a standard curve is generated by seeding different amount of OVCAR-3 cells (based on counting with a hemacytometer) in a 96-well plate, and measuring genomic DNA abundance after overnight incubation. Three independent experiments are performed and data is pooled for statistical analysis[2]. |
Source |
Synonyms | Kempferol, Robigenin |
Molecular Weight | 286.24 |
Formula | C15H10O6 |
CAS No. | 520-18-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 28.6 mg/mL (100 mM)
DMSO: 28.6 mg/mL (100 mM)
You can also refer to dose conversion for different animals. More
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Kaempferol 520-18-3 Apoptosis Autophagy Endocrinology/Hormones Metabolism Microbiology/Virology Proteases/Proteasome Estrogen Receptor/ERR Mitophagy Estrogen/progestogen Receptor HIV Protease Endogenous Metabolite Parasite inhibit Kempferol Human immunodeficiency virus HIV Mitochondrial Autophagy Inhibitor Robigenin inhibitor