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Kaempferol

Catalog No. T2177   CAS 520-18-3
Synonyms: Kempferol, Robigenin

Kaempferol (Robigenin) is a natural flavonoid and an inverse agonist of ERRα and ERRγ. Kaempferol has a wide range of antitumor, anti-inflammatory, antioxidant, antibacterial and antiviral activities.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Kaempferol Chemical Structure
Kaempferol, CAS 520-18-3
Pack Size Availability Price/USD Quantity
50 mg In stock $ 46.00
100 mg In stock $ 58.00
200 mg In stock $ 91.00
500 mg In stock $ 197.00
1 mL * 10 mM (in DMSO) In stock $ 50.00
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Purity: 99.41%
Purity: 99.11%
Purity: 99.09%
Purity: 98%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Kaempferol (Robigenin) is a natural flavonoid and an inverse agonist of ERRα and ERRγ. Kaempferol has a wide range of antitumor, anti-inflammatory, antioxidant, antibacterial and antiviral activities.
In vitro Kaempferol, a natural flavonoid found extensively in fruits and vegetables, exhibits powerful anti-inflammatory properties by suppressing interleukin-4 and cyclo-oxygenase 2 expression through the inhibition of Src kinase activity and downregulation of the NFκB pathway. It effectively halts angiogenesis and promotes the apoptosis of ovarian cancer cells. Notably, long-term consumption of kaempferol has been associated with a significantly reduced risk of ovarian cancer among American female nurses, according to prospective studies. In tests on ovarian cancer cell lines, kaempferol demonstrated a significant, concentration-dependent reduction in cell proliferation following a 24-hour exposure, particularly at concentrations of 40 μM or higher. Besides its anti-cancer effects, kaempferol also markedly inhibits NADPH oxidase activity, reducing reactive oxygen species (ROS) by directly binding to NADPH oxidase, and protects against Ang II-induced sinus nodal cell death by reducing CAMKII oxidation. Additionally, in sensitized RBL-2H3 cells, kaempferol at doses of 10-20 μM suppresses the release of inflammatory agents and attenuates the activation of Syk and PLCγ, as well as reduces COX2 induction in response to allergen challenge, demonstrating its broad-spectrum biological activities.
In vivo COX2 induction was confirmed in the airways of BSA-challenged BALB/c mice, while untreated control mice displayed a lack of COX2. Inhalation of BSA led to an enhanced induction of COX2 (dark brown staining) in the mouse airway, which was reversed by the oral administration of Kaempferol. Additionally, marked goblet cell hyperplasia and epithelial thickening were observed in BSA-challenged mice; however, these symptoms were completely alleviated with the supplementation of 20 mg/kg Kaempferol[4].
Kinase Assay Right atria or sinus nodal cells are homogenized in lysis buffer consisting of (50 mM Tris-HCl pH 7.5, 100 mM KCl, 1 mM ethylenediamine tetraacetic acid, 1 mM ethylene glycol tetraacetic acid, 1 mM dithiothreitol, 0.1 mM phenylmethylsulfonyl fluoride, 0.5 mM Benzamidine, 20 mg/L Leupeptin, 20 mM sodium pyrophosphate, 50 mM NaF, and 50 mM sodium β-glycerophosphate), and total protein content is determined by the Bradford assay. Caspase-3 activity is determined by EnzChek Caspase-3 Assay Kit[3].
Cell Research Kaempferol is prepared in DMSO (100 mM) and stored (-20°C), and then diluted with appropriate medium[2]. Ovarian cancer cells are seeded in 96-well plates at 2000 cells/well and incubated overnight before treatment with 0-160 μM Kaempferol for 24 hours in triplicates. The medium is removed, and the plates are freeze-thawed to lyse cells. Each well is added with 200 μL 1× CyQUANT cell lysis buffer containing 5x SYBR Green I and incubated at room temperature (RT) for 5 minutes. The reaction (50 μL) is transferred to PCR strip tubes and the fluorescent signal is measured at 90°C with a real-time Chromo4 PCR instrument. To ensure that cell proliferation assays are performed within a linear range of cell numbers, a standard curve is generated by seeding different amount of OVCAR-3 cells (based on counting with a hemacytometer) in a 96-well plate, and measuring genomic DNA abundance after overnight incubation. Three independent experiments are performed and data is pooled for statistical analysis[2].
Source
Synonyms Kempferol, Robigenin
Molecular Weight 286.24
Formula C15H10O6
CAS No. 520-18-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 28.6 mg/mL (100 mM)

DMSO: 28.6 mg/mL (100 mM)

TargetMolReferences and Literature

1. Zhu L, et al. Kaempferol Suppresses Proliferation and Induces Cell Cycle Arrest, Apoptosis, and DNA Damage in Breast Cancer Cells. Oncol Res. 2019 Jun 21;27(6):629-634. 2. Kim TW, et al. Kaempferol induces autophagic cell death via IRE1-JNK-CHOP pathway and inhibition of G9a in gastric cancer cells. Cell Death Dis. 2018 Aug 29;9(9):875. 3. Yang QS, et al. Kaempferol pretreatment modulates systemic inflammation and oxidative stress following hemorrhagic shock in mice. Chin Med. 2015 Mar 21;10:6. 4. Cheng X, et al. Kaempferol alleviates LPS-induced neuroinflammation and BBB dysfunction in mice via inhibiting HMGB1 release and down-regulating TLR4/MyD88 pathway. Int Immunopharmacol. 2018 Mar;56:29-35. 5. Hwang Y H, Jang S A, Kim T, et al. Anti-osteoporotic and Anti-adipogenic Effects of Rhus chinensis Nutgalls in Ovariectomized Mice Fed with a High-fat Diet[J]. Planta medica. 2019. 6. Shim K S, Hwang Y H, Jang S A, et al. . Water Extract of Lysimachia christinae Inhibits Trabecular Bone Loss and Fat Accumulation in Ovariectomized Mice[J]. Nutrients.  2020, 12(7): 1927.

TargetMolCitations

1. Wang R, Deng Z, Zhu Z, et al.Kaempferol promotes non-small cell lung cancer cell autophagy via restricting Met pathway.Phytomedicine.2023: 155090. 2. Shao Y, Tang B, Ding Y, et al.Kaempferide ameliorates cisplatin-induced nephrotoxicity via inhibiting oxidative stress and inducing autophagy.Acta Pharmacologica Sinica.2023: 1-13. 3. Shan H, Zhang X, Mi Y, et al.Eriodictyol Suppresses Gastric Cancer Cells via Inhibition of PI3K/AKT Pathway.Pharmaceuticals.2022, 15(12): 1477. 4. Shim K S, Hwang Y H, Jang S A, et al.  Water Extract of Lysimachia christinae Inhibits Trabecular Bone Loss and Fat Accumulation in Ovariectomized Mice. Nutrients. 2020, 12(7): 1927 5. Hwang Y H, Jang S A, Kim T, et al. Anti-osteoporotic and Anti-adipogenic Effects of Rhus chinensis Nutgalls in Ovariectomized Mice Fed with a High-fat Diet. Planta Medica. 2019, 85(14/15): 1128-1135 6. Jang S A, Hwang Y H, Yang H, et al. Ethanolic extract of Pyrrosia lingua (Thunb.) Farw. ameliorates OVX-induced bone loss and RANKL-induced osteoclastogenesis. Biomedicine & Pharmacotherapy. 2022, 147: 112640. 7. Shan H, Zhang X, Mi Y, et al.Eriodictyol Suppresses Gastric Cancer Cells via Inhibition of PI3K/AKT Pathway.Pharmaceuticals.2022, 15(12): 1477. 8. Zhang Y, Yu F, Hao J, et al. Study on the Effective Material Basis and Mechanism of Traditional Chinese Medicine Prescription (QJC) Against Stress Diarrhea in Mice. Frontiers in Veterinary Science. 2021, 8. 9. Lee S J, Yang H, Kim S C, et al.Ethanol Extract of Radix Asteris Suppresses Osteoclast Differentiation and Alleviates Osteoporosis.International Journal of Molecular Sciences.2023, 24(22): 16526. 10. Du Y Y, Sun T, Yang Q, et al.Therapeutic Potential of Kaempferol against Sleep Deprivation-Induced Cognitive Impairment: Modulation of Neuroinflammation and Synaptic Plasticity Disruption in Mice.ACS Pharmacology & Translational Science.2023
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Related compound libraries

This product is contained In the following compound libraries:
Miao medicine Compound Library Tobacco Monomer Library Antiparasitic Natural Product Library Anti-Cancer Active Compound Library Anti-Parasitic Compound Library Natural Product Library for HTS Anti-Infection Compound Library Anti-Aging Compound Library FDA-Approved & Pharmacopeia Drug Library Natural Product Library

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Keywords

Kaempferol 520-18-3 Apoptosis Autophagy Endocrinology/Hormones Metabolism Microbiology/Virology Proteases/Proteasome Estrogen Receptor/ERR Mitophagy Estrogen/progestogen Receptor HIV Protease Endogenous Metabolite Parasite inhibit Kempferol Human immunodeficiency virus HIV Mitochondrial Autophagy Inhibitor Robigenin inhibitor

 

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