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ML264

Catalog No. T6894   CAS 1550008-55-3
Synonyms: CID-51003603

ML264 (CID-51003603) is a selective kruppel-like factor 5 (KLF5) inhibitor, which potently Inhibits growth of Colorectal Cancer.

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ML264 Chemical Structure
ML264, CAS 1550008-55-3
Pack Size Availability Price/USD Quantity
1 mg In stock $ 32.00
5 mg In stock $ 67.00
10 mg In stock $ 102.00
25 mg In stock $ 208.00
50 mg In stock $ 363.00
100 mg In stock $ 547.00
1 mL * 10 mM (in DMSO) In stock $ 67.00
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Purity: 99.45%
Purity: 98.72%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description ML264 (CID-51003603) is a selective kruppel-like factor 5 (KLF5) inhibitor, which potently Inhibits growth of Colorectal Cancer.
Targets&IC50 KLF5:29 nM
In vitro ML264 potently halts DLD-1 viability (IC50 = 29 nM) with high maximal effect (>90%). DLD-1 cells are human colorectal adenocarcinoma cells. ML264 has significant effects at submicromolar doses on other cell types as well, including HCT116 (human colorectal carcinoma), HT29 (human colorectal adenocarcinoma), and SW620 (human colorectal adenocarcinoma). The IEC-6 anti-target (a nontransformed rat intestinal epithelial cell line) is largely unaffected, with inhibition below 50% at the highest dose[1].This compound potently inhibits proliferation of CRC cells in vitro through modifications of the cell cycle profile[2].
In vivo In an established xenograft mouse model of colon cancer, ML264 efficiently inhibits growth of the tumor within five days of treatment. this effect is caused by a significant reduction in proliferation and that ML264 potently inhibits the expression of KLF5 and EGR1, a transcriptional activator of KLF5[2].
Cell Research For cell proliferation experiments, DLD-1 and HCT116 cells are treated with 10μM ML264 or with vehicle (DMSO). Live cells are collected at 24, 48 and 72 hours post treatment and their numbers are determined by counting using a Coulter counter; In MTS assay, DLD-1 and HCT116 cells are treated with 10μM ML264 or with vehicle (DMSO). After 24, 48, and 72 hours of incubations, 20 μL of MTS solution  is added to each well and an analysis is performed according to the manufacturer's protocol. (Only for Reference)
Synonyms CID-51003603
Molecular Weight 384.88
Formula C17H21ClN2O4S
CAS No. 1550008-55-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 71 mg/mL (184.5 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

TargetMolReferences and Literature

1. Bialkowska A,et al. Probe Reports from the NIH Molecular Libraries Program. 2010-2011 Oct 31 [updated 2013 Mar 7]. 2. Ruiz de Sabando A, et al. Mol Cancer Ther. 2016, 15(1):72-83.

TargetMolCitations

1. Shen X, Zhang Y, Xu Z, et al. KLF5 inhibition overcomes oxaliplatin resistance in patient-derived colorectal cancer organoids by restoring apoptotic response. Cell Death & Disease. 2022, 13(4): 1-13

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Active Compound Library Covalent Inhibitor Library Stem Cell Differentiation Compound Library Anti-Aging Compound Library HIF-1 Signaling Pathway Compound Library NO PAINS Compound Library Anti-Liver Cancer Compound Library Toxic Compound Library Inhibitor Library DNA Damage & Repair Compound Library

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Keywords

ML264 1550008-55-3 Cell Cycle/Checkpoint DNA Damage/DNA Repair MAPK DNA/RNA Synthesis KLF ML-264 CID-51003603 Inhibitor Krüppel-like factor inhibit ML 264 CID 51003603 CID51003603 inhibitor

 

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