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PAC-1

Catalog No. T2503 CAS 315183-21-2

Synonyms: Procaspase activating compound 1

PAC-1 has been used in trials studying the treatment of Lymphoma, Melanoma, Solid Tumors, Breast Cancer, and Thoracic Cancers, among others.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

PAC-1, CAS 315183-21-2

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	PAC-1 has been used in trials studying the treatment of Lymphoma, Melanoma, Solid Tumors, Breast Cancer, and Thoracic Cancers, among others.
Targets&IC50	Procaspase-3:0.22 μM(EC50)
Kinase Assay	In vitro procaspase-3 activation: Procaspase-3 is expressed and purified in Escherichia coli. Various concentrations of PAC-1 are added to 90 μL of a 50 ng/mL of procaspase-3 in caspase assay buffer in a 96-well plate, The plate is incubated for 12 hours at 37 °C. A 10 μL volume of a 2 mM solution of caspase-3 peptidic substrate acetyl Asp-Glu-Val-Asp-p-nitroanilide (Ac-DEVD-pNa) in caspase assay buffer is then added to each well. The plate is read every 2 minutes at 405 nm for 2 hours in a Spectra Max Plus 384 well plate reader. The slope of the linear portion for each well is determined, and the relative increase in activation from untreated control wells is calculated.
Cell Research	Cells are exposed to various concentrations of PAC-1 for 72 hours. Cell death is quantified by the addition of MTS/PMS CellTiter 96 Cell Proliferation Assay reagent. The plates are incubated at 37 °C for approximately 1 hour (until the colored product formed), and the absorbance is measured at 490 n(Only for Reference)

Synonyms	Procaspase activating compound 1
Molecular Weight	392.49
Formula	C23H28N4O2
CAS No.	315183-21-2

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

1eq. HCl: 29.4 mg/mL (75 mM)

DMSO: 39.3 mg/mL (100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Putt KS, et al. Nat Chem Biol, 2006, 2(10), 543-550. 2. Peterson QP, et al. J Mol Biol, 2009, 388(1), 144-158. 3. Seervi M, et al. Cell Death Dis, 2011, 2, e207.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

PAC-1 315183-21-2 Apoptosis Autophagy Proteases/Proteasome Caspase PAC 1 Inhibitor PAC1 Procaspase activating compound-1 inhibit Procaspase activating compound 1 Procaspase activating compound1 inhibitor

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