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PFK-015

Catalog No. T2455 CAS 4382-63-2

Synonyms: PFK15, PFK 015

PFK-015 (PFK15) is an effective inhibitor of PFKFB3 (IC50: 110 nM) and inhibits PFKFB3 activity in Y cells (IC50: 20 nM).

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PFK-015, CAS 4382-63-2

Pack Size	Availability	Price/USD
5 mg	In stock	$ 52.00
10 mg	In stock	$ 81.00
25 mg	In stock	$ 162.00
50 mg	In stock	$ 297.00
100 mg	In stock	$ 441.00
200 mg	In stock	$ 612.00
500 mg	In stock	$ 976.00
1 mL * 10 mM (in DMSO)	In stock	$ 52.00

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Purity: 99.7%

Purity: 98.94%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	PFK-015 (PFK15) is an effective inhibitor of PFKFB3 (IC50: 110 nM) and inhibits PFKFB3 activity in Y cells (IC50: 20 nM).
Targets&IC50	PFKFB3:110 nM, PFKFB3:20 nM
In vitro	PFK-015 (25 mg/kg, i.p.) inhibits the growth, metastatic spread, and glucose metabolism of LLC tumors in syngeneic mice. In three distinct athymic mouse models of xenografted human cancers, the anticancer efficacy of PFK-015 is comparable to that of established chemotherapy drugs. PFK-015 exhibits favorable pharmacokinetic properties in vivo.
In vivo	PFK-015 effectively reduces levels of F26BP, glucose uptake, and intracellular adenosine triphosphate (ATP) in Jurkat T-cell leukemia cells and H522 lung adenocarcinoma cells. Additionally, PFK-015 exhibits significant inhibitory effects on the growth of a variety of cancer cells.
Kinase Assay	Recombinant PFKFB3 assay: Kinase reactions are conducted by incubating 13 ng of recombinant human PFKFB3 protein in a reaction mix containing 10 μmol/L ATP, 10 μmol/L F6P, and either dimethyl sulfoxide (DMSO) vehicle control, 3PO, or PFK15 for 1 hour at room temperature. Kinase activity is measured with the Adapta Universal Kinase Assay per manufacturer
Cell Research	Viability is determined using trypan blue exclusion. Cells were incubated in 20% trypan blue for 5 minutes. Cells excluding trypan blue are counted using a standard hemocytometer to determine the total number of viable cells. Experiments are conducted in triplicate. (Only for Reference)

Synonyms	PFK15, PFK 015
Molecular Weight	260.29
Formula	C17H12N2O
CAS No.	4382-63-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 26 mg/mL (100 mM)

References and Literature

1. Clem BF, et al. Mol Cancer Ther. 2013, 12(8), 1461-1470. 2. Zhang Y, Wang X, Li X, et al. Synergistic effect of colistin combined with PFK-158 against colistin-resistant Enterobacteriaceae[J]. Antimicrobial agents and chemotherapy. 2019, 63(7): e00271-19.

Citations

1. Zhang Y, Wang X, Li X, et al. Synergistic effect of colistin combined with PFK-158 against colistin-resistant Enterobacteriaceae. Antimicrobial Agents and Chemotherapy. 2019, 63(7): e00271-19

Related compound libraries

This product is contained In the following compound libraries：

Kinase Inhibitor Library Inhibitor Library Target-Focused Phenotypic Screening Library Metabolism Compound Library Bioactive Compound Library Autophagy Compound Library Apoptosis Compound Library ReFRAME Related Library Bioactive Compounds Library Max Glycometabolism Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

PFK-015 4382-63-2 Autophagy Metabolism Glucokinase PFK-15 PFK15 3PO stomach cancer PFK 015 cancers inhibit PFK 15 PFKFB3 esophageal squamous cell carcinoma (ESCC) PFK015 lung cancer colon cancer Inhibitor inhibitor

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