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Product Introduction

Bioactivity

Description	Acarbose (BAY g 5421) is an inhibitor of α-Glucosidases with antihyperglycemic activity.
In vitro	Acarbose binds to and inhibits α-glucosidase, an enteric enzyme found in the brush border of the small intestines that hydrolyzes oligosaccharides and disaccharides into glucose and other monosaccharides. In addition, acarbose inhibits pancreatic α-amylase which hydrolyzes complex starches to oligosaccharides in the small intestines.
Cell Research	Cell viability is determined using the MTT assay. Cells are seeded in 24-well culture plates at a density of 2×104 cells/well, incubated for 48 h, treated with acarbose at varying concentrations (0.5, 1.0, 2.0, 3.0, and 5.0 μM) for 24 h; or pre-treated with TNF-α (20 ng/mL) for either 24 h or 48 h to evaluate the dose-dependent effects of acarbose on VSMC growth and viability, cultured with 0.5 mg/mL MTT at 37°C in a humidified atmosphere of 5% CO2 for another 4 h, and solubilized with isopropanol. The viable cell number varies directly with the concentration of formazan product measured spectrophotometrically at 563 nm.
Alias	BAY g 5421

Chemical Properties

Molecular Weight	645.6
Formula	C₂₅H₄₃NO₁₈
Cas No.	56180-94-0

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 50 mg/mL (77.45 mM)

H2O: 64.5 mg/mL (100 mM)

Solution Preparation Table

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	1.5489 mL	7.7447 mL	15.4895 mL	77.4473 mL
5 mM	0.3098 mL	1.5489 mL	3.0979 mL	15.4895 mL
10 mM	0.1549 mL	0.7745 mL	1.5489 mL	7.7447 mL
20 mM	0.0774 mL	0.3872 mL	0.7745 mL	3.8724 mL
50 mM	0.0310 mL	0.1549 mL	0.3098 mL	1.5489 mL

H2O

	1mg	5mg	10mg	50mg
100 mM	0.0155 mL	0.0774 mL	0.1549 mL	0.7745 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

mellitusoralantihyperglycemicinsulinGlucosidaseanti-diabetesBAY g5421InhibitorBAY g-5421inhibitAcarbose