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Product Introduction

Bioactivity

Description	Apramycin sulfate (Nebramycin II) is an aminoglycoside antibiotic.
In vitro	Compared to untreated chicks, 225 mg/L Apramycin completely inhibited mortality and significantly reduced the excretion of Salmonella.
In vivo	At 1/4 MIC, apramycin reduces the growth rate of the test strains in the culture medium and induces a post-antibiotic effect lasting up to 5 hours. It inhibits A. pleuropneumoniae FMV 87-682 with an MIC50 of 8 mg/l. Apramycin significantly decreases the hemolytic activity of A. pleuropneumoniae and affects capsule production in this isolate and in a P. multocida isolate (type A). Misreading induced by apramycin, involving the incorporation of leucine, isoleucine, and serine, was detected, albeit limited to a narrow scope, in an E. coli cell-free system programmed with poly(U).
Alias	Nebramycin II, Ai3-29795

Chemical Properties

Molecular Weight	637.66
Formula	C₂₁H₄₃N₅O₁₅S
Cas No.	65710-07-8

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

H2O: 10 mM

DMSO: Insoluble

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	1.5682 mL	7.8412 mL	15.6823 mL	78.4117 mL
5 mM	0.3136 mL	1.5682 mL	3.1365 mL	15.6823 mL
10 mM	0.1568 mL	0.7841 mL	1.5682 mL	7.8412 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Apramycin sulfateinhibitAntibioticInhibitorApramycin SulfateApramycinBacterial