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Biotin

🥰Excellent
Catalog No. T1116Cas No. 58-85-5
Alias Vitamin H, Vitamin B7, D-Biotin

Biotin (Vitamin H) is a water-soluble B-vitamin and is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids.

Biotin

Biotin

🥰Excellent
Purity: 100%
Catalog No. T1116Alias Vitamin H, Vitamin B7, D-BiotinCas No. 58-85-5
Biotin (Vitamin H) is a water-soluble B-vitamin and is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids.
Pack SizePriceAvailabilityQuantity
500 mg$46In Stock
1 g$72In Stock
5 g$271In Stock
1 mL x 10 mM (in DMSO)$48In Stock
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Product Introduction

Bioactivity
Description
Biotin (Vitamin H) is a water-soluble B-vitamin and is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids.
In vitro
METHODS: To induce significant biotinylation of neighboring proteins, supplementation with Biotin is required at concentrations typically between 10-50 µM, with optimal incubation times between 15-18 h, after which total biotinylation plateaus.
RESULTS: Due to the formation of covalent bonds between biotin and lysine residues on proteins, biotinylation appears to remain constant throughout the life cycle of the protein and can withstand stringent cleavage and washing conditions, thus maximizing the purity of isolated proteins identified by MS. The identified proteins can then be investigated as candidate interactors with POIs. [1]
METHODS: Mouse oligodendrocytes 158N were treated with Biotin (1-1000 nM) for 2 h, followed by the addition of 7β-OHC (50 µM) for a further 24 h. Cell viability was measured by MTT Assay.
RESULTS: 7β-OHC-induced cytotoxicity was only prevented by Biotin at a concentration of 10-1000 nM. [2]
In vivo
Biotin serves as a coenzyme for carboxylases, involved in the synthesis of fatty acids, isoleucine, and valine, as well as in gluconeogenesis. In biological research, it is primarily used to label proteins for biochemical studies.
AliasVitamin H, Vitamin B7, D-Biotin
Chemical Properties
Molecular Weight244.31
FormulaC10H16N2O3S
Cas No.58-85-5
SmilesC(CCCC(O)=O)[C@H]1[C@@]2([C@@](NC(=O)N2)(CS1)[H])[H]
Relative Density.1.267 g/cm3
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4.6 mg/mL (18.83 mM), In vivo: Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 50 mg/mL (204.66 mM)
Solution Preparation Table
DMSO
1mg5mg10mg50mg
20 mM0.2047 mL1.0233 mL2.0466 mL10.2329 mL
50 mM0.0819 mL0.4093 mL0.8186 mL4.0932 mL
100 mM0.0409 mL0.2047 mL0.4093 mL2.0466 mL

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Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
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