Powder: -20°C for 3 years | In solvent: -80°C for 1 year
iCRT3 is a Wnt and β-catenin-responsive transcription inhibitor.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
2 mg | In stock | $ 39.00 | |
5 mg | In stock | $ 64.00 | |
10 mg | In stock | $ 97.00 | |
25 mg | In stock | $ 187.00 | |
50 mg | In stock | $ 293.00 | |
100 mg | In stock | $ 478.00 | |
500 mg | In stock | $ 1,050.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 70.00 |
Description | iCRT3 is a Wnt and β-catenin-responsive transcription inhibitor. |
Targets&IC50 | Wnt/β-catenin:8.2 nM |
In vitro | Treatment with iCRT3 at doses of 12.5, 25, 50, and 75 μM decreases TNF-α levels by 14.7%, 18.5%, 44.9% and 61.3%, respectively. |
In vivo | The IL-6 levels in the 10?mg/kg iCRT3 treatment mice are lower. |
Kinase Assay | In the determination of full-length baculovirus expressed Syk kinase activity, the reaction system was 25 μL containing 25 mM Tris-HCl, pH 7.5, 5 mM β-glycerophosphate, 2 mM DTT, 0.1 mM Na3VO4, 10 mM MgCl2, 0.5 μM Promega PTK Biotin Peptide Substrate 1, 0.01% casein, 1, 0.01% Triton X-100, 0.25% Glycerol, and 40 mM ATP (Km for ATP). After incubation for 60 minutes at room temperature, the reaction was stopped by adding 30 mM EDTA (30 μL of SA-APC and 4 nM PT-66 antibody). After measuring the plate, the IC50 value of the test compound was calculated using a 4-parameter linear regression algorithm. |
Cell Research | Cells are seeded into 96-well plates to a density of 5×103 cells per well and incubated in the culture medium with iCRT3 for an additional 48 h. Cell viability and cell apoptosis assays are carried out using a Cell Counting kit-8 and a Caspase-Glo 3/7 assay kit according to the manufacturer's instructions, respectively. |
Animal Research | NOD-SCID BALB/c mice inoculated A172 cells are treated with iCRT3 (5 mg/kg) diluted in PBS i.p. triweekly. |
Molecular Weight | 394.53 |
Formula | C23H26N2O2S |
CAS No. | 901751-47-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 150 mg/mL
You can also refer to dose conversion for different animals. More
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iCRT3 901751-47-1 Apoptosis Cytoskeletal Signaling Stem Cells Wnt/beta-catenin Inhibitor Wnt inhibit iCRT-3 iCRT 3 inhibitor